caa a22 4500 445840706 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0171-3 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0171-3 Sulfur starvation and restoration affect nitrate uptake and assimilation in rapeseed [Elektronische Daten] [Gurjeet Kaur, Ruby Chandna, Renu Pandey, Yash Abrol, Muhammad Iqbal, Altaf Ahmad] We analyzed the effect of omission of sulfur (S) from the nutrient solution and then restoration of S-source on the uptake and assimilation of nitrate in rapeseed. Incubation in nutrient solution without S for 1-6days led to decline in uptake of nitrate, activities, and expression levels of nitrate reductase (NR) and glutamine synthetase (GS). The nitrite reductase (NiR) and glutamate synthase (GOGAT) activities were not considerably affected. There was significant enhancement in nitrate content and decline in sulfate content. Evaluation of amino acid profile under S-starvation conditions showed two- to fourfold enhancement in the contents of arginine, asparagine and O-acetyl-l-serine (OAS), whereas the contents of cysteine and methionine were reduced heavily. When the S-starved plants were subjected to restoration of S for 1, 3, 5, and 7days, activities and expression levels of NR and GS recovered within the fifth and seventh days of restoration, respectively. Exogenous supply of metabolites (arginine, asparagine, cysteine, glutamine, OAS, and methionine) also affected the uptake and assimilation of nitrate, with a maximum for OAS. These results corroborate the tight interconnection of S-nutrition with nitrate assimilation and that OAS plays a major role in this regulation. The study must be helpful in developing a nutrient-management technology for optimization of crop productivity. Springer-Verlag, 2010 Brassica rapa L. (rapeseed) nationallicence Nitrate assimilation nationallicence Nitrate uptake nationallicence Sulfur restoration nationallicence Sulfur starvation nationallicence Kaur Gurjeet Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Chandna Ruby Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Pandey Renu Division of Plant Physiology, Indian Agricultural Research Institute, 110012, New Delhi, India aut Abrol Yash Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Iqbal Muhammad Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Ahmad Altaf Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Protoplasma Springer Vienna 248/2(2011-04-01), 299-311 0033-183X 248:2<299 2011 248 709 https://doi.org/10.1007/s00709-010-0171-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0171-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaur Gurjeet Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Chandna Ruby Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Pandey Renu Division of Plant Physiology, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Abrol Yash Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Iqbal Muhammad Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Ahmad Altaf Molecular Ecology Laboratory, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 299-311 0033-183X 248:2<299 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer