caa a22 4500 445840722 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0166-0 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0166-0 Subcellular localization of V2 protein of Tomato leaf curl Java virus by using green fluorescent protein and yeast hybrid system [Elektronische Daten] [Pradeep Sharma, Rajarshi Gaur, Masato Ikegami] Tomato leaf curl Java virus-A (ToLCJV-A[ID]) from Southeast Asia is a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. Previously, we have elucidated the role of V1 ORF encoded by ToLCJV-A[ID] in cell-to-cell movement. In this study, the role of V2 (PreCP) in localization was determined. Subcellular localization of ToLCJV-A[ID] V2 in plant tissues showed that this protein is co-localized to the cell cytoplasm, perinuclear and associated with the endoplasmic reticulum network. The results obtained from deletion analysis indicate that fusion of N-terminal part of the V2, containing the nuclear export signals (NES), directed the accumulation of fluorescence towards the cell cytoplasm. Furthermore, functionality of the NES (20LAVKYLQLV29) in the N-terminal part of the V2 protein was confirmed by one-hybrid yeast system. Taken together, these results suggest that V2 enhances the coat protein-mediated nuclear export of ToLCJV-A[ID] and is consistent with the model in which V2 mediates viral DNA export from the nucleus to the plasmodesmata. Springer-Verlag, 2010 Begomovirus nationallicence Subcellular localization nationallicence GFP nationallicence V2 protein nationallicence Sharma Pradeep Graduate School of Agricultural Science, Tohoku University, Sendai, Japan aut Gaur Rajarshi Mody Institute of Science and Technology, Sikar, Rajasthan, India aut Ikegami Masato Graduate School of Agricultural Science, Tohoku University, Sendai, Japan aut Protoplasma Springer Vienna 248/2(2011-04-01), 281-288 0033-183X 248:2<281 2011 248 709 https://doi.org/10.1007/s00709-010-0166-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0166-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma Pradeep Graduate School of Agricultural Science, Tohoku University, Sendai, Japan aut NATIONALLICENCE 700 1- Gaur Rajarshi Mody Institute of Science and Technology, Sikar, Rajasthan, India aut NATIONALLICENCE 700 1- Ikegami Masato Graduate School of Agricultural Science, Tohoku University, Sendai, Japan aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 281-288 0033-183X 248:2<281 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer