caa a22 4500 445840749 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0177-x doi (NATIONALLICENCE)springer-10.1007/s00709-010-0177-x The reaction of Lupinus angustifolius L. root meristematic cell nucleoli to lead [Elektronische Daten] [Łucja Balcerzak, Sława Glińska, Mirosław Godlewski] The effect of 2-48h treatment of Lupinus angustifolius L. roots with lead nitrate at the concentration of 10−4M on the nucleoli in meristematic cells was investigated. In the lead presence the number of ring-shaped as well as segregated nucleoli increased especially after 12-48h of treatment, while spindle-shaped nucleoli appeared after 24h and 48h. Lead presence also increased the frequency of cells with silver-stained particles in the nucleus and the number of these particles especially from the 12th hour of treatment. It was accompanied by significant decline of nucleolar area. Analysis of these cells in transmission electron microscope confirmed the presence of ring-shaped and segregated nucleoli. Moreover, electron microscopy revealed compact structure nucleoli without granular component. Additionally, one to three oval-shaped fibrillar structures attached to nucleolus or lying free in the nucleoplasm were visible. The possible mechanism of lead toxicity to the nucleolus is briefly discussed. Springer-Verlag, 2010 Lead nationallicence Stress nationallicence Nucleolus nationallicence Root meristem nationallicence Lupinus angustifolius L nationallicence Balcerzak Łucja Department of Plant Cytology and Cytochemistry, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut Glińska Sława Laboratory of Electron Microscopy, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut Godlewski Mirosław Department of Plant Cytology and Cytochemistry, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut Protoplasma Springer Vienna 248/2(2011-04-01), 353-361 0033-183X 248:2<353 2011 248 709 https://doi.org/10.1007/s00709-010-0177-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0177-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balcerzak Łucja Department of Plant Cytology and Cytochemistry, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut NATIONALLICENCE 700 1- Glińska Sława Laboratory of Electron Microscopy, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut NATIONALLICENCE 700 1- Godlewski Mirosław Department of Plant Cytology and Cytochemistry, University of Lodz, Banacha 12/16, 91-227, Lodz, Poland aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 353-361 0033-183X 248:2<353 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer