caa a22 4500 445840757 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0202-0 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0202-0 Development and application of probes for labeling the actin cytoskeleton in living plant cells [Elektronische Daten] [Fei Du, Haiyun Ren] The actin cytoskeleton is one of the most important components of eukaryotic cytoskeletons. It participates in numerous crucial procedures of cells and has been studied by using various methods. The development and application of appropriate probes for actin visualization is the first and foremost step for functional analysis of actin in vivo. Since the actin cytoskeleton is a highly dynamic and sensitive structure, methods previously used to visualize actin often harm cells and cannot reveal the native state of the actin cytoskeleton in living cells. The development of labeling technologies for living plant cells, especially the emergence and application of green fluorescent protein-tagged actin markers, has provided new insights into the structure and function of the actin cytoskeleton in vivo. There has been a number of probes for actin labeling in living plant cells though they each present different advantages and defects. In this review, we discuss and compare those widely used methods for actin visualization and analysis. Springer-Verlag, 2010 Actin cytoskeleton nationallicence Labeling method nationallicence Fluorescent probes nationallicence Living plant cell nationallicence Du Fei Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, and College of Life Science, Beijing Normal University, 100875, Beijing, China aut Ren Haiyun Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, and College of Life Science, Beijing Normal University, 100875, Beijing, China aut Protoplasma Springer Vienna 248/2(2011-04-01), 239-250 0033-183X 248:2<239 2011 248 709 https://doi.org/10.1007/s00709-010-0202-0 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0202-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Du Fei Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, and College of Life Science, Beijing Normal University, 100875, Beijing, China aut NATIONALLICENCE 700 1- Ren Haiyun Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, and College of Life Science, Beijing Normal University, 100875, Beijing, China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 239-250 0033-183X 248:2<239 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer