caa a22 4500 445840781 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0162-4 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0162-4 Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype [Elektronische Daten] [Xin Guan, Heqing Zhao, Yan Xu, Yuejin Wang] Vitis pseudoreticulata glyoxal oxidase (VpGLOX) was previously isolated from the Chinese wild vine V. pseudoreticulata accession "Baihe-35-1” during a screen for genes that are upregulated in response to infection with grapevine powdery mildew (Erysiphe necator, PM). In the present study, a possible function of VpGLOX for defense against PM was investigated using Agrobacterium-mediated transient expression. After optimizing agro-infiltration, VpGLOX was transiently overexpressed in leaves of either PM-susceptible (accession "6-12-2”) or PM-resistant (accession "6-12-6”) plants. The efficiency of transfection was verified using a β-glucuronidase (GUS) reporter and was found to comprise most leaf areas regardless of the initial leaf position. Upon infection with E. necator, clear differences were observed with respect to hyphal development between agro-infiltrated leaves and control groups of both, the susceptible and the resistant, genotypes. The expression of VpGLOX was followed by real-time polymerase chain reaction in both genotypes. Whereas in the susceptible host ("6-12-2”) expression was found to increase only in transfected leaves and remained transient, in the resistant host ("6-12-6”), a second peak appeared later in transfected leaves, probably representing the response of the endogenous VpGLOX. The data support the interpretation that VpGLOX is sufficient to confer resistance to E. necator. Springer-Verlag, 2010 Glyoxal oxidase nationallicence Grapevine nationallicence Hyphal development nationallicence Transient expression nationallicence Erysiphe necator nationallicence Guan Xin College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Zhao Heqing College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Xu Yan College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Wang Yuejin College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut Protoplasma Springer Vienna 248/2(2011-04-01), 415-423 0033-183X 248:2<415 2011 248 709 https://doi.org/10.1007/s00709-010-0162-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0162-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guan Xin College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Heqing College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yan College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yuejin College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, People's Republic of China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 415-423 0033-183X 248:2<415 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer