caa a22 4500 44584079X CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0167-z doi (NATIONALLICENCE)springer-10.1007/s00709-010-0167-z The dynamics of the actin cytoskeleton during sporogenesis in Psilotum nudum L [Elektronische Daten] [Dorota Tchórzewska, Józef Bednara] The actin cytoskeleton (microfilaments, MFs) accompanies the tubulin cytoskeleton (microtubules) during the meiotic division of the cell, but knowledge about the scope of their physiological competence and cooperation is insufficient. To cast more light on this issue, we analysed the F-actin distribution during the meiotic division of the Psilotum nudum sporocytes. Unfixed sporangia of P. nudum were stained with rhodamine-phalloidin and 4′,6-diamidino-2-phenylindole dihydrochloride, and we monitored the changes in the actin cytoskeleton and nuclear chromatin throughout sporogenesis. We observed that the actin cytoskeleton in meiotically dividing cells is not only part of the kariokinetic spindle and phragmoplast but it also forms a well-developed network in the cytoplasm present in all phases of meiosis. Moreover, in telophase I F-actin filaments formed short-lived phragmoplast, which was adjacent to the plasma membrane, exactly at the site of future cell wall formation. Additionally, the meiocytes were pre-treated with cytochalasin-B at a concentration that causes damage to the MFs. This facilitated observation of the effect of selective MFs damage on the course of meiosis and sporogenesis of P. nudum. Changes were observed that occurred in the cytochalasin-treated cells: the daughter nuclei were located abnormally close to each other, there was no formation of the equatorial plate of organelles and, consequently, meiosis did not occur normally. It seems possible that, if the actin cytoskeleton only is damaged, regular cytokinesis will not occur and, hence, no viable spores will be produced. The Author(s), 2010 F-actin nationallicence Meiosis nationallicence Rhodamine-phalloidin nationallicence Cytochalasin-B nationallicence Psilotum nudum nationallicence Tchórzewska Dorota Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut Bednara Józef Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut Protoplasma Springer Vienna 248/2(2011-04-01), 289-298 0033-183X 248:2<289 2011 248 709 https://doi.org/10.1007/s00709-010-0167-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0167-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tchórzewska Dorota Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Bednara Józef Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 289-298 0033-183X 248:2<289 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer