caa a22 4500 445840811 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0180-2 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0180-2 Actin filament-organized local cortical endoplasmic reticulum aggregations in developing stomatal complexes of grasses [Elektronische Daten] [Eleni Giannoutsou, Panagiotis Apostolakos, Basil Galatis] Endoplasmic reticulum (ER) immunolabeling in developing stomatal complexes and in the intervening cells of the stomatal rows (ICSRs) of Zea mays revealed that the cortical-ER forms distinct aggregations lining locally expanding wall regions. The polarized subsidiary cell mother cells (SMCs), displayed a cortical-ER-patch lining the wall region shared with the inducing guard cell mother cell (GMC), which disorganized during mitosis. In dividing SMCs, ER persisted in the preprophase band region and was unequally distributed in the mitotic spindle poles. The subsidiary cells (SCs) formed initially an ER-patch lining the common wall with the GMC or the young guard cells and afterwards an ER-ring in the junction of the SC wall with the neighboring ones. Distinct ER aggregations lined the ICSR wall regions shared with the SCs. The cortical-ER aggregations in stomatal cells of Z. mays were co-localized with actin filament (AF) arrays but both were absent from the respective cells of Triticum turgidum, which follow a different morphogenetic pattern. Experimental evidence showed that the interphase ER aggregations are organized by the respective AF arrays, while the mitotic ER aggregations by microtubules. These results revealed that AF and ER demarcated "cortical cytoplasmic domains” are activated below the locally expanding stomatal cell wall regions, probably via a mechanosensing mechanism triggered by the locally stressed plasmalemma/cell wall continuum. The probable role(s) of the local ER aggregations are discussed. Springer-Verlag, 2010 Actin filaments nationallicence Endoplasmic reticulum nationallicence Stomatal complexes nationallicence Triticum turgidum nationallicence Zea mays nationallicence AF : Actin filament nationallicence but-1 : Butanol-1 nationallicence but-2 : Butanol-2 nationallicence ER : Endoplasmic reticulum nationallicence GC : Guard cell nationallicence GMC : Guard cell mother cell nationallicence ICSR : Intervening cell of the stomatal row nationallicence lat-B : Latrunculin-B nationallicence MT : Microtubule nationallicence PLC : Phospholipase-C nationallicence PLD : Phospholipase -D nationallicence PPB : Preprophase band nationallicence SC : Subsidiary cell nationallicence SMC : Subsidiary cell mother cell nationallicence Giannoutsou Eleni Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut Apostolakos Panagiotis Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut Galatis Basil Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut Protoplasma Springer Vienna 248/2(2011-04-01), 373-390 0033-183X 248:2<373 2011 248 709 https://doi.org/10.1007/s00709-010-0180-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0180-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giannoutsou Eleni Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut NATIONALLICENCE 700 1- Apostolakos Panagiotis Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut NATIONALLICENCE 700 1- Galatis Basil Department of Botany, Faculty of Biology, University of Athens, 15784, Athens, Greece aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 373-390 0033-183X 248:2<373 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer