caa a22 4500 44584082X CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0160-6 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0160-6 Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine [Elektronische Daten] [Huiling Wang, Wei Wang, Hui Li, Ping Zhang, Jicheng Zhan, Weidong Huang] Anthocyanidin synthase (ANS) is one of the key enzymes in the biosynthesis of both anthocyanins and proanthocyanidins in grapevine. Although substantial researches have investigated ANS gene expression and regulation at the transcriptional level, little is yet known about protein expression and distribution in grapevine. Here, the expression and tissue and subcellular localization of ANS in different Cabernet sauvignon grapevine tissues were investigated by using the techniques of Western blotting, immunohistochemical localization, immuno-electron microscopy, and confocal microscopy. The results showed that the ANS was expressed in the grape berries, leaves, stems, petioles, and leaf buds. In grape berry skin and flesh, ANS expression is developmental dependent. Immunohistochemical analysis revealed that ANS is primarily distributed in the exocarp, mesocarp, and seed of the fruit; in palisade and spongy tissues of the leaves; in the primary phloem and pith ray in the stems; and in the growth point and leaf primordium of the leaf buds. Furthermore, at the subcellular level, the ANS was mainly localized in the cytoplasm regardless of cell types and some ANS were also found in the nucleus in the mesocarp vascular bundle and leaf bud cells. This research will give further insight for the biosynthesis and regulation of different flavonoid compounds in grapevine. Springer-Verlag, 2010 Anthocyanidin synthase nationallicence Grapevine nationallicence Tissue localization nationallicence Subcellular localization nationallicence Transient expression nationallicence ANR : Anthocyanidin reductase nationallicence ANS : Anthocyanidin synthase nationallicence BSA : Bovine serum albumin nationallicence F3′5′H : Flavonoid 3′, 5′-hydroxylase nationallicence IgG : Immunoglobulin fractions nationallicence PAs : Proanthocyanidins nationallicence PBS : Phosphate-buffered saline nationallicence RSR : Regular salt rinse solution nationallicence Wang Huiling College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Wang Wei College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Li Hui College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Zhang Ping College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Zhan Jicheng College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Huang Weidong College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut Protoplasma Springer Vienna 248/2(2011-04-01), 267-279 0033-183X 248:2<267 2011 248 709 https://doi.org/10.1007/s00709-010-0160-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0160-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Huiling College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Wang Wei College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Li Hui College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Zhang Ping College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Zhan Jicheng College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Huang Weidong College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 267-279 0033-183X 248:2<267 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer