caa a22 4500 445840870 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10725-010-9541-2 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9541-2 In vitro and ex vitro evaluation of long-term micropropagated turmeric as analyzed through cytophotometry, phytoconstituents, biochemical and molecular markers [Elektronische Daten] [Sanghamitra Nayak, Tanzeer Kaur, Sujata Mohanty, Goutam Ghosh, Rakesh Choudhury, Laxmikanta Acharya, Enketeswara Subudhi] Turmeric (Curcuma longa L.), a high valued medicinal plant, was micropropagated through induction of multiple shoots using latent axillary buds of rhizome. Cytophotometric and random amplified polymorphic DNA (RAPD) as well as inter simple sequence repeats (ISSR) analysis were used to periodically monitor the genetic stability of micropropagated clones of Curcuma longa conserved in vitro up to 7years at every 6months interval. A total of eighteen RAPD and eight ISSR primers gave 45,537 distinct and reproducible bands, monomorphic across all 353 plants analyzed. Micropropagated turmeric after being conserved for 7years in vitro was transplanted into soil in field. Drug yielding potential of tissue culture derived plants was evaluated in field through estimation of phytoconstituents like curcumin and essential oil contents. The result of 2years of field trial showed that micropropagated turmeric retained stability in all the characteristics examined when compared with the field performance of conventionally propagated plants. Thus long term conservation of an elite genotype of turmeric with epigenetic and genetic stability is significant for stable supply of drug i.e., curcumin and essential oil to the market. Springer Science+Business Media B.V., 2010 Curcuma longa nationallicence Drug yield nationallicence Micropropagation nationallicence Stability nationallicence Nayak Sanghamitra Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut Kaur Tanzeer Jaypee University of Information Technology, Waknaghat, Solan, Simla, Himachal Pradesh, India aut Mohanty Sujata Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut Ghosh Goutam School of Pharmaceutical Sciences, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut Choudhury Rakesh Jaypee University of Information Technology, Waknaghat, Solan, Simla, Himachal Pradesh, India aut Acharya Laxmikanta Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut Subudhi Enketeswara Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 91-98 0167-6903 64:1<91 2011 64 10725 https://doi.org/10.1007/s10725-010-9541-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9541-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nayak Sanghamitra Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut NATIONALLICENCE 700 1- Kaur Tanzeer Jaypee University of Information Technology, Waknaghat, Solan, Simla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Mohanty Sujata Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut NATIONALLICENCE 700 1- Ghosh Goutam School of Pharmaceutical Sciences, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut NATIONALLICENCE 700 1- Choudhury Rakesh Jaypee University of Information Technology, Waknaghat, Solan, Simla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Acharya Laxmikanta Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut NATIONALLICENCE 700 1- Subudhi Enketeswara Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, 751003, Bhubaneswar, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 91-98 0167-6903 64:1<91 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer