caa a22 4500 445840919 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10725-010-9533-2 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9533-2 Cloning, characterization, and expression of the BjEXPA1 gene and its promoter region from Brassica juncea L [Elektronische Daten] [Tao Sun, Yuxiu Zhang, Tuanyao Chai] To explore the regulation of expansin expression under heavy metal stresses, a novel expansin gene, BjEXPA1, and its upstream region were obtained from Indian mustard (Brassica juncea L.). BjEXPA1 encodes a cell wall protein of the α-subgroup of the expansin family. The expression of BjEXPA1 is regulated by exogenous phytohormones, NaCl, and heavy metals. Sequence analysis of its upstream region revealed the presence of several putative cis-acting elements, including phytohormone response, abiotic stress response, and tissue-specific elements. GUS activity under the control of the BjEXPA1 promoter allowed observation of BjEXPA1 spatial and temporal expression patterns. The reporter construct indicated that BjEXPA1 is induced by exogenous gibberellin and auxin, and inhibited by abscisic acid, CdCl2 and PEG6000. Transgenic tobacco seedlings overexpressing BjEXPA1 showed an altered phenotype and enhanced sensitivity to cadmium. Higher H2O2 accumulation and extensive plasma membrane damage were detected in the leaves of transgenic seedlings when exposed to Cd, compared with wild-type plants. Our findings support the conclusion that overexpression of BjEXPA1 in tobacco renders plants susceptible to Cd stress, probably due to disruption of the growth process and/or the breakdown of cell walls. Springer Science+Business Media B.V., 2010 Expansin nationallicence Heavy metal nationallicence Promoter nationallicence Growth phenotype nationallicence GUS : β-glucuronidase nationallicence SIMR : Stress-induced morphogenic response nationallicence X-Gluc : 5-bromo-4-chloro-3-indolyl-glucronide nationallicence SA : Salicylic acid nationallicence ABA : Abscisic acid nationallicence GA3 : Gibberellin nationallicence RACE : Rapid amplification of cDNA ends nationallicence GFP : Green fluorescent protein nationallicence IAA : Indole-3-acetic acid nationallicence PEG 6000 : Polyethylene glycol 6000 nationallicence MUG : 4-methyl umbelliferyl glucuronide nationallicence H2O2 : Hydrogen peroxide nationallicence MDA : Malondialdehyde nationallicence POD : Peroxidase nationallicence EL : Electrolyte leakage nationallicence ORF : Open reading frame nationallicence pI : Isoelectric point nationallicence Sun Tao College of Life Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, 100049, Beijing, China aut Zhang Yuxiu School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), 11 Xueyuan Road, 100083, Beijing, China aut Chai Tuanyao College of Life Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, 100049, Beijing, China aut Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 39-51 0167-6903 64:1<39 2011 64 10725 https://doi.org/10.1007/s10725-010-9533-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9533-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Tao College of Life Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, 100049, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yuxiu School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), 11 Xueyuan Road, 100083, Beijing, China aut NATIONALLICENCE 700 1- Chai Tuanyao College of Life Science, Graduate University of Chinese Academy of Sciences, Yuquan Road 19, 100049, Beijing, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 39-51 0167-6903 64:1<39 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer