caa a22 4500 445840927 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10725-010-9526-1 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9526-1 Manganese-excess induces oxidative stress, lowers the pool of antioxidants and elevates activities of key antioxidative enzymes in rice seedlings [Elektronische Daten] [Sarita Srivastava, R. Dubey] Manganese (Mn) is an essential element for plant growth but in excess, specially in acidic soils, it can become phytotoxic. In order to investigate whether oxidative stress is associated with the expression of Mn toxicity during early seedling establishment of rice plants, we examined the changes in the level of reactive oxygen species (ROS), oxidative stress induced an alteration in the level of non-enzymic antioxidants and activities of antioxidative enzymes in rice seedlings grown in sand cultures containing 3 and 6mM MnCl2. Mn treatment inhibited growth of rice seedlings, the metal increasingly accumulated in roots and shoots and caused damage to membranes. Mn treated plants showed increased generation of superoxide anion (O2 .−), elevated levels of H2O2 and thiobarbituric acid reactive substances (TBARS) and decline in protein thiol. The level of nonprotein thiol, however, increased due to Mn treatment. A decline in contents of reduced ascorbate (AsA) and glutathione (GSH) as well as decline in ratios of their reduced to oxidize forms was observed in Mn-treated seedlings. The activities of antioxidative enzymes superoxide dismutase (SOD) and its isoforms Mn SOD, Cu/Zn SOD, Fe SOD as well as guaiacol peroxidase (GPX) increased in the seedlings due to Mn treatment however, catalase (CAT) activity increased in 10days old seedlings but it declined by 20days under Mn treatment. The enzymes of Halliwell-Asada cycle, ascorbate peroxidase (APX) monodehydoascorbate reductase (MDHAR), dehyroascorbate reductase (DHAR) and glutathione reductase (GR) increased significantly in Mn treated seedlings over controls. Results suggest that in rice seedlings excess Mn induces oxidative stress, imbalances the levels of antioxidants and the antioxidative enzymes SOD, GPX, APX and GR appear to play an important role in scavenging ROS and withstanding oxidative stress induced by Mn. Springer Science+Business Media B.V., 2010 Antioxidants nationallicence Antioxidative defense system nationallicence Manganese nationallicence Oryza sativa L nationallicence Oxidative stress nationallicence Reactive oxygen species nationallicence Rice nationallicence Srivastava Sarita Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 1-16 0167-6903 64:1<1 2011 64 10725 https://doi.org/10.1007/s10725-010-9526-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9526-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srivastava Sarita Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/1(2011-05-01), 1-16 0167-6903 64:1<1 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer