caa a22 4500 445840951 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-010-9544-z doi (NATIONALLICENCE)springer-10.1007/s10725-010-9544-z Wang Yu Department of Pharmaceutical Biology, Monash University, 381 Royal Parade, VIC 3052, Parkville, Melbourne, Australia aut An efficient protocol for stimulating cell development in protoplast culture of S caevola [Elektronische Daten] [Yu Wang] Scaevola, characterised by unique fan-shaped flowers, is an Australian endemic ornamental having a great commercial potential. The breeding of Scaevola however is limited due to poor seed germination, therefore it is critical to understand the embryogenesis in Scaevola so as to facilitate its breeding improvement programs. Direct differentiation of embryo structures was first reported here in mesophyll protoplast cultures of Scaevola aemula. The isolated protoplasts initiated cell division when cultured in KM or MS medium. Higher plating efficiencies were observed in the medium containing a combination of NAA and BAP in contrast to 2,4-D and BAP. The formation of globular embryo structures was successfully achieved. This protoplast culture system can be utilized as an experimental platform for the study of embryogenic differentiation of cells. It may open new vistas to investigate the seed development at molecular and cellular levels in Scaevola and related Australian native plants that are well known for their low seed viability and germination. 9Springer Science+Business Media B.V., 2010 Protoplast culture nationallicence Embryogenesis nationallicence Dividing efficiency nationallicence Plating efficiency nationallicence BAP : 6-Benzylaminopurine nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence NAA : α-Naphthaleneacetic acid nationallicence Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 293-299 0167-6903 64:3<293 2011 64 10725 https://doi.org/10.1007/s10725-010-9544-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9544-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Wang Yu Department of Pharmaceutical Biology, Monash University, 381 Royal Parade, VIC 3052, Parkville, Melbourne, Australia aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 293-299 0167-6903 64:3<293 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer