caa a22 4500 44584096X CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-010-9542-1 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9542-1 Effects of 5-aminolevulinic acid on growth and amylase activity in the radish taproot [Elektronische Daten] [Masakazu Hara, Ikuo Takahashi, Michiyo Yamori, Toru Tanaka, Shigeyuki Funada, Keitaro Watanabe] 5-Aminolevulinic acid (ALA) promotes the growth of plants by enhancing their photosynthetic activities, but there is little information on how ALA influences the metabolism of sugars produced by photosynthesis. Here, we report the effects of ALA on tissue growth, sugar content, and amylase activity in the radish taproot. 5-Aminolevulinic acid was applied with a foliar spray (5.3-13,500μM), and application at concentrations of 53, 530, and 2,700μM enhanced the fresh weight of the taproot. Glucose is a major soluble sugar of the radish taproot. 5-Aminolevulinic acid slightly increased the glucose content but did not influence the fructose, sucrose, or starch contents. Radishes have β-amylase (RsBAMY1), which is expressed in the taproot. 5-Aminolevulinic acid enhanced both the amylase activity and the RsBAMY1 protein accumulation. These results suggest that ALA may control starch accumulation by increasing the RsBAMY1 expression in the radish taproot. The relationship between taproot growth and free sugar accumulation by ALA is also discussed. Springer Science+Business Media B.V., 2010 5-Aminolevulinic acid nationallicence β-Amylase (EC 3.2.1.2) nationallicence Plant growth nationallicence Raphanus sativus nationallicence ALA : 5-aminolevulinic acid nationallicence DAS : Days after sowing nationallicence RsBAMY1 : Raphanus sativus β-amylase 1 nationallicence Hara Masakazu Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut Takahashi Ikuo Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut Yamori Michiyo Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut Tanaka Toru Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut Funada Shigeyuki Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut Watanabe Keitaro Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 287-291 0167-6903 64:3<287 2011 64 10725 https://doi.org/10.1007/s10725-010-9542-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9542-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hara Masakazu Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut NATIONALLICENCE 700 1- Takahashi Ikuo Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut NATIONALLICENCE 700 1- Yamori Michiyo Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Suruga-ku, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tanaka Toru Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut NATIONALLICENCE 700 1- Funada Shigeyuki Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut NATIONALLICENCE 700 1- Watanabe Keitaro Research and Development Center, Cosmo Oil Co., Ltd., 1134-2, Gongendo, 340-0193, Satte-Shi, Saitama, Japan aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 287-291 0167-6903 64:3<287 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer