caa a22 4500 445840978 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-011-9566-1 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9566-1 Nickel and Al-excess inhibit nitrate reductase but upregulate activities of aminating glutamate dehydrogenase and aminotransferases in growing rice seedlings [Elektronische Daten] [Pallavi Mishra, R. Dubey] The present study was undertaken to examine the influence of toxic levels of Ni and Al, on the activities of key nitrogen assimilatory enzymes in roots and shoots of growing rice seedlings. When seedlings of two inbred rice (Oryza sativa L.) cvs. Malviya-36 and Pant-12, sensitive to both Ni and Al, were raised in sand cultures containing 200 and 400μM NiSO4 or 80 and 160μM Al2(SO4)3, a marked inhibition in the activities of NO3 − assimilatory enzymes NR and GS was observed in roots as well as shoots during a 5-20day growth period. Both Ni and Al treatments, in growth medium, stimulated the activity of aminating glutamate dehydrogenase (NADH-GDH) whereas the activity of deaminating GDH (NAD+-GDH) decreased under metal toxicities. The activities of the aminotransferases studied; alanine aminotransferase (AlaAT) and aspartate amino transferase (AspAT) increased due to Ni and Al treatments. Results suggest that both Ni and Al treatments impair N assimilation in rice seedlings by inhibiting the activities of NR and GS and that GDH appears to play a role in assimilation of NH4 + in metal stress conditions. Further, higher activity of aminotransferases in metal stressed seedlings might be helpful in meeting higher demand of amino acids under stressed conditions. Springer Science+Business Media B.V., 2011 Nickel nationallicence Aluminum nationallicence Toxicity nationallicence Nitrogen assimilation nationallicence Oryza sativa L nationallicence Mishra Pallavi Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 251-261 0167-6903 64:3<251 2011 64 10725 https://doi.org/10.1007/s10725-011-9566-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9566-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishra Pallavi Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 251-261 0167-6903 64:3<251 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer