caa a22 4500 445840994 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-011-9563-4 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9563-4 Characterization of oxidative and antioxidative events during dehydration and rehydration of resurrection plant Ramonda nathaliae [Elektronische Daten] [Živko Jovanović, Tamara Rakić, Branka Stevanović, Svetlana Radović] In order to investigate changes of oxidative status in relation to the activity of the various protective mechanisms in resurrection plant Ramonda nathaliae, we have analysed time and relative water content (RWC) related changes in lipid peroxidation and ion leakage, hydrogen peroxide accumulation, changes of pigment content and antioxidative enzyme activity, together with expression of dehydrins. The results indicate that enhanced oxidative status during dehydration, not previously reported for resurrection plants, could play an active role in inducing the desiccation adaptive response in R. nathaliae. A critical phase is shown to exist during dehydration (in the range of RWC between 50 and 70%) during which a significant increase in hydrogen peroxide accumulation, lipid peroxidation and ion leakage, accompanied by a general decline in antioxidative enzyme activity, takes place. This phase is designated as a transition characterized by change in the type of stress response. The initial response, relying mainly on the enzymatic antioxidative system, is suspended but more effective, desiccation specific protective mechanisms, such as expression of dehydrins, are then switched on. The expression of dehydrins in R. nathaliae could be inducible as well as constitutive. In order to cope with the oxidative stress associated with rapid rewatering, R. nathaliae reactivated antioxidative enzymes. We propose that controlled elevation of reactive oxygen species, such as hydrogen peroxide, could be an important mechanism enabling resurrection plants to sense dehydration and to trigger an adaptive programme at an appropriate stage during the dehydration/rehydration cycle. Springer Science+Business Media B.V., 2011 Resurrection plants nationallicence Ramonda nathaliae nationallicence Dehydration/rehydration nationallicence Antioxidative systems nationallicence Jovanović Živko Plant Molecular Biology Lab, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O. Box 23, 11 010, Belgrade, Serbia aut Rakić Tamara Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11 000, Belgrade, Serbia aut Stevanović Branka Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11 000, Belgrade, Serbia aut Radović Svetlana Faculty of Biology, Department of Biochemistry and Molecular Biology, Institute of Physiology and Biochemistry, University of Belgrade, Studentski trg 3/II, 11 000, Belgrade, Serbia aut Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 231-240 0167-6903 64:3<231 2011 64 10725 https://doi.org/10.1007/s10725-011-9563-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9563-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jovanović Živko Plant Molecular Biology Lab, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O. Box 23, 11 010, Belgrade, Serbia aut NATIONALLICENCE 700 1- Rakić Tamara Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11 000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Stevanović Branka Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Takovska 43, 11 000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Radović Svetlana Faculty of Biology, Department of Biochemistry and Molecular Biology, Institute of Physiology and Biochemistry, University of Belgrade, Studentski trg 3/II, 11 000, Belgrade, Serbia aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 231-240 0167-6903 64:3<231 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer