caa a22 4500 44584101X CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-010-9559-5 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9559-5 Chilling-induced changes of vacuolar proton pumps in hypocotyls of Vigna unguiculata [Elektronische Daten] [Luciana Oliveira, Alana Sobreira, José Costa, Maria de Otoch, Masayoshi Maeshima, Dirce Fernandes de Melo] Vigna unguiculata (cowpea) is a legume adapted to high temperatures and is sensitive to low temperatures. Temperature is one of the limiting factors of growth and yield for many crops but its effect on cowpea metabolism is not known. We investigated the effect of chilling on activity of vacuolar proton pumps (V-ATPase and V-PPase) and their protein content in tonoplast vesicles of cowpea hypocotyls. Seedlings grown for 7days at 10 or 4°C were used for experiments. Chilling treatment at 10 or 4°C markedly suppressed growth of cowpea seedlings. Following chilling at 10 and 4°C, activity of both proton pumps and the relative amount of V-PPase and subunit A of V-ATPase were significantly increased. Both substrate hydrolysis and H+ transport activities of V-PPase remained at relatively high levels during chilling treatment. For V-ATPase, treatment at 10°C for 6days increased the ATP hydrolysis activity. However, the H+ transport activity of the enzyme was increased when treated for 4days but was markedly decreased when treated for 6days. Our results provide evidence for different regulation for these vacuolar proton pumps, indicating that V-PPase is the more stable proton pump throughout chilling stress. Springer Science+Business Media B.V., 2011 Chilling nationallicence Cowpea nationallicence Stress nationallicence Vacuole nationallicence V-ATPase nationallicence V-PPase nationallicence ACMA : 9-amino-6-chloro-2-methoxyacridine nationallicence BTP : Bis-tris-propano(1,3-bis(tris(hydroxymethyl)methylamino)-propane) nationallicence DTT : Dithiothreitol nationallicence D : Days nationallicence EDTA : Ethylenediaminetetra-acetic acid nationallicence EGTA : Ethylene glycol-bis (β-aminoethyl ether) N, N, N′, N′-tetraacetic acid nationallicence PMSF : Phenylmethyl-sulfonyl fluoride nationallicence V-ATPase : Vacuolar H+-ATPase nationallicence V-PPase : Vacuolar H+-translocating pyrophosphatase nationallicence Oliveira Luciana Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut Sobreira Alana Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut Costa José Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut Otoch Maria de Departamento de Biologia, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil aut Maeshima Masayoshi Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan aut Fernandes de Melo Dirce Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 211-219 0167-6903 64:3<211 2011 64 10725 https://doi.org/10.1007/s10725-010-9559-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9559-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oliveira Luciana Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut NATIONALLICENCE 700 1- Sobreira Alana Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut NATIONALLICENCE 700 1- Costa José Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut NATIONALLICENCE 700 1- Otoch Maria de Departamento de Biologia, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil aut NATIONALLICENCE 700 1- Maeshima Masayoshi Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan aut NATIONALLICENCE 700 1- Fernandes de Melo Dirce Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal, 6029, CEP-60.455-760, Fortaleza, Ceará, Brazil aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 211-219 0167-6903 64:3<211 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer