caa a22 4500 445841044 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10725-010-9550-1 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9550-1 Identification of the tomato ABA-deficient mutant sitiens as a member of the ABA-aldehyde oxidase gene family using genetic and genomic analysis [Elektronische Daten] [E. Harrison, A. Burbidge, J. Okyere, A. Thompson, I. Taylor] The sitiens (sit) wilty mutant of tomato (Solanum lycopersicum L.) is deficient in functional enzyme activity at the final step in abscisic acid (ABA) biosynthesis. The biochemical lesion is believed to be an impaired aldehyde oxidase (AO). Molecular mapping using various interspecies crosses has previously shown sit to co-map with a cluster of unresolved RFLP markers on the short arm of chromosome 1. Here, the utilisation of bridging lines to produce interspecies mapping populations involving a self-compatible S. peruvianum accession (LA2157) allowed the fine mapping of sit within this cluster. Identification of a novel AO gene, within the region now known to contain the sit locus, was confirmed by analysis of the tomato whole genome shotgun sequence assembly. This novel AO protein shares 76-78% identity at the amino acid level with the previously characterised tomato AO proteins. The DNA sequence of this putative sit gene was characterised in wild type and in two allelic sit mutants (sit and sit w): changes in DNA sequence were identified in these mutant alleles that cause a truncation of exon 2 and the deletion of exon 7, respectively. These results establish the identity of the tomato sit gene and are consistent with its proposed function of encoding the ABA aldehyde oxidase apoenzyme. Springer Science+Business Media B.V., 2010 Tomato nationallicence Sitiens nationallicence Abscisic acid nationallicence Aldehyde oxidase nationallicence Molecular mapping nationallicence Harrison E. Warwick-HRI, University of Warwick, CV35 9EF, Wellesbourne, Warwickshire, UK aut Burbidge A. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut Okyere J. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut Thompson A. Warwick-HRI, University of Warwick, CV35 9EF, Wellesbourne, Warwickshire, UK aut Taylor I. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 301-309 0167-6903 64:3<301 2011 64 10725 https://doi.org/10.1007/s10725-010-9550-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9550-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harrison E. Warwick-HRI, University of Warwick, CV35 9EF, Wellesbourne, Warwickshire, UK aut NATIONALLICENCE 700 1- Burbidge A. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut NATIONALLICENCE 700 1- Okyere J. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut NATIONALLICENCE 700 1- Thompson A. Warwick-HRI, University of Warwick, CV35 9EF, Wellesbourne, Warwickshire, UK aut NATIONALLICENCE 700 1- Taylor I. Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leicestershire, UK aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/3(2011-07-01), 301-309 0167-6903 64:3<301 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer