caa a22 4500 445841060 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10725-010-9553-y doi (NATIONALLICENCE)springer-10.1007/s10725-010-9553-y Cytokinin and abscisic acid control plastid gene transcription during barley seedling de-etiolation [Elektronische Daten] [Alexander Kravtsov, Yan Zubo, Maria Yamburenko, Olga Kulaeva, Victor Kusnetsov] The regulatory role of cytokinins and abscisic acid (ABA) during de-etiolation of monocotyledonous plants is not sufficiently studied. The effects of cytokinin—6-benzyladenine (BA) and ABA on leaf greening and chloroplast gene transcription were studied on leaves detached from etiolated barley (Hordeum vulgare L.) seedlings. The influence of both phytohormones was age-dependent. Cytokinin practically had no effect on the youngest (3-day-old) leaves, increased chlorophyll accumulation in 6-day-old leaves, and this effect decreased in 9-day-old leaves. ABA reduced chlorophyll accumulation in the youngest leaves, and this effect was blocked by cytokinin. ABA alone had practically no effect on greening of leaves detached from 6- and 9-day-old seedlings, but fully inhibited activation of this process by cytokinin. Thus, antagonism between ABA and cytokinin was observed for seedlings of all ages. The rate of transcription of chloroplast genes was studied by run-on transcription in isolated chloroplasts in the course of etiolated seedling growth in darkness, and age-dependent decrease was demonstrated. It was established that cytokinin activated transcription of 11 chloroplast genes among 30 studied, and ABA suppressed transcription of 12 plastid genes. Such differential effect of cytokinin and ABA on chloroplast gene transcription in monocot seedlings during etiolated leaf greening was not known before. Springer Science+Business Media B.V., 2010 Cytokinin nationallicence Abscisic acid nationallicence Chloroplast transcription nationallicence ABA : Abscisic acid nationallicence BA : 6-benzyladenine nationallicence SE : Standard error nationallicence Kravtsov Alexander Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Zubo Yan Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Yamburenko Maria Institute of Biology, Humboldt University, Chausseestrasse 117, 10115, Berlin, Germany aut Kulaeva Olga Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Kusnetsov Victor Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 173-183 0167-6903 64:2<173 2011 64 10725 https://doi.org/10.1007/s10725-010-9553-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9553-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kravtsov Alexander Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Zubo Yan Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Yamburenko Maria Institute of Biology, Humboldt University, Chausseestrasse 117, 10115, Berlin, Germany aut NATIONALLICENCE 700 1- Kulaeva Olga Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 700 1- Kusnetsov Victor Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 173-183 0167-6903 64:2<173 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer