caa a22 4500 445841079 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10725-010-9556-8 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9556-8 The brachytic 2 and 3 maize double mutant shows alterations in plant growth and embryo development [Elektronische Daten] [Elena Cassani, Daniele Villa, Massimo Durante, Michela Landoni, Roberto Pilu] In maize there are three types of brachytic mutants (br1, br2 and br3) showing short stature and a gibberellins-insensitive phenotype. So far only the brachytic 2 gene has been cloned and it encodes for a putative protein of the Multidrug Resistant (MDR) class of P-glycoproteins (PGPs) that could be involved in polar movement of auxins: in fact the br2 mutant is insensitive to treatment with auxins and gibberellins. We have isolated a new recessive mutation of br2 gene (named br2-23) and with the aim of study its interactions with the other brachytic mutations we produced a br2 br3 double mutant that showed an additive effect on the stature with respect to the single mutants br2 and br3 and abnormal growth. In the progeny of the selfed double mutant we observed various defective seedlings, mirroring an altered embryo development and growth, which also suggested a role for the br3 gene in auxin transport. Expression analysis of the auxin efflux transporters codified by ZmPIN1 genes supports this finding, showing the up-regulation of the ZmPIN1a gene in the br3 mutant. To our knowledge this is the first report showing the involvement of Br2 and Br3 genes in embryo development. These single and double mutants appear to be useful tools to study the genetics of plant height and to investigate auxin transport in plants. Springer Science+Business Media B.V., 2010 Maize nationallicence Embryo development nationallicence Brachytic 2 gene nationallicence Polar auxin transport nationallicence ZmPIN1 genes nationallicence Cassani Elena Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut Villa Daniele Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut Durante Massimo Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut Landoni Michela Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Milan, Italy aut Pilu Roberto Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 185-192 0167-6903 64:2<185 2011 64 10725 https://doi.org/10.1007/s10725-010-9556-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9556-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cassani Elena Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Villa Daniele Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Durante Massimo Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Landoni Michela Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Pilu Roberto Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 185-192 0167-6903 64:2<185 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer