caa a22 4500 445841095 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10725-010-9546-x doi (NATIONALLICENCE)springer-10.1007/s10725-010-9546-x Isolation and characterization of a harvest-induced promoter of an alfalfa gene, hi7 [Elektronische Daten] [Jian Zhang, Wen-Jin Yu, Ai-Sheng Xiong, Bahman Bahramnejad, Larry Erickson] A cDNA library was made by subtractive hybridization of cDNA from non-harvested and harvested alfalfa plants. One cDNA clone, hi7, in Northern blot analysis showed a high level of transcription of this gene in harvested tissue, but not in non-harvested tissue, nor in wounded or heat shock treated tissue. A 640bp promoter region isolated by genomic walking based on hi7 cDNA sequence, was fused to a GUS gene and transferred to tobacco and Medicago truncatula. When leaf tissue from transgenic tobacco plants was harvested, incubated for 24h at room temperature, and then stained for GUS activity, a strong blue colour was observed throughout the tissue, but not in tissue that was stained immediately following harvesting. The expression of the GUS gene was also induced in tobacco plants sprayed with abscissic acid (ABA) and in callus derived from these plants when transferred to medium containing ABA. Springer Science+Business Media B.V., 2010 Harvest-induced gene nationallicence hi7 nationallicence Promoter nationallicence ABA nationallicence Transgenic plants nationallicence Alfalfa nationallicence Zhang Jian Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Yu Wen-Jin Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Xiong Ai-Sheng Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China aut Bahramnejad Bahman Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Erickson Larry Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 119-128 0167-6903 64:2<119 2011 64 10725 https://doi.org/10.1007/s10725-010-9546-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9546-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jian Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Yu Wen-Jin Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Xiong Ai-Sheng Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China aut NATIONALLICENCE 700 1- Bahramnejad Bahman Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Erickson Larry Plant Agriculture Department, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 119-128 0167-6903 64:2<119 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer