caa a22 4500 44584115X CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10725-010-9557-7 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9557-7 The role of calcium in regulating alginate-derived oligosaccharides in nitrogen metabolism of Brassica campestris L. var. utilis Tsen et Lee [Elektronische Daten] [Yunhong Zhang, Geng Zhang, Lanying Liu, Kai Zhao, Lishu Wu, Chengxiao Hu, Hongjie Di] Under normal (full-strength) levels of calcium in hydroponics culture, alginate-derived oligosaccharides (ADOs) increased the activity of nitrate reductase (NR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), and endpeptidase (EP) in leaves by 4.04-14.41%, 23.57-190.12%, 103.44-265.21%, and 7.22-157.41% respectively compared to that in leaves of the control plants; increased the concentration of NH4 +-N, total nitrogen, and protein nitrogen in leaves; and decreased the concentration of nitrate in shoots. Under low (5% of the full strength) levels of calcium, ADOs had no effect on the activity of NR and GS. These results serve to confirm the role of ADOs in regulating nitrogen metabolism, which is related to the supply of calcium. ADOs induce the release of calcium from cell organelles and the cell wall into the cytoplasm to activate the enzymes involved in nitrogen metabolism. Thus Ca regulates nitrogen metabolism in Chinese cabbage through ADOs. Springer Science+Business Media B.V., 2010 Alginate-derived oligosaccharide nationallicence Flowering Chinese cabbage ( Brassica campestris L . var . utilis Tsen et Lee) nationallicence Nitrogen metabolism nationallicence Calcium nationallicence ADOs : Alginate-derived oligosaccharides nationallicence ATP : Adenosine triphosphate nationallicence EDTA-2Na : Ethylene diamine tetraacetic acid disodium salt nationallicence EP : Endopeptidase nationallicence GDH : Glutamate dehydrogenase nationallicence GOGAT : Glutamate synthetase nationallicence GS : Glutamine synthetase nationallicence NADH : Nicotinamide-adenine dinucleotid nationallicence NADPH : Nicotinamide adenine dinucleotide phosphate nationallicence NH4 +-N : Ammonia nitrogen nationallicence NO3 −-N : Nitrate nitrogen nationallicence NR : Nitrate reductase nationallicence Zhang Yunhong Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Zhang Geng Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Liu Lanying Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Zhao Kai Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Wu Lishu Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Hu Chengxiao Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut Di Hongjie Centre for Soil and Environment Research, Lincoln University, Lincoln, P.O. Box 84, 7647, Christchurch, New Zealand aut Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 193-202 0167-6903 64:2<193 2011 64 10725 https://doi.org/10.1007/s10725-010-9557-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9557-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yunhong Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Geng Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Liu Lanying Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhao Kai Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Wu Lishu Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Hu Chengxiao Microelement Research Centre, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Di Hongjie Centre for Soil and Environment Research, Lincoln University, Lincoln, P.O. Box 84, 7647, Christchurch, New Zealand aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 193-202 0167-6903 64:2<193 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer