caa a22 4500 445841176 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10725-010-9548-8 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9548-8 In vitro plant regeneration from organogenic callus of Curcuma kwangsiensis Lindl. (Zingiberaceae) [Elektronische Daten] [Shijun Zhang, Nian Liu, Aiwu Sheng, Guohua Ma, Guojiang Wu] A novel protocol for callus-mediated shoot regeneration was established for an important medicinal and ornamental plant native to South China, Curcuma kwangsiensis, using shoot base sections excised from seedlings in vitro as explant sources. The frequency of callus formation reached 91% for explants cultured on MS medium containing 1.4μM TDZ, 4.4μM BA and 2.3μM 2,4-D. 8.2 shoots per callus was achieved on MS medium supplemented with 1.4μM TDZ, 17.8μM BA and 2.7μM NAA. Single shoots transferred into MS medium free of plant growth regulator rooted well. Regenerated plants acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse conditions. Springer Science+Business Media B.V., 2010 Curcuma kwangsiensis nationallicence Rhizome nationallicence Plant regeneration nationallicence Callus nationallicence BA : 6 Benzylaminopurine nationallicence NAA : Naphtaleneacetic acid nationallicence 2,4-D : 2,4 Dichlorophenoxyacetic acid nationallicence TDZ : Thidiazuron nationallicence PGR : Plant growth regulator nationallicence Zhang Shijun South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut Liu Nian College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, 510225, Guangzhou, China aut Sheng Aiwu College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, 510225, Guangzhou, China aut Ma Guohua South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut Wu Guojiang South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 141-145 0167-6903 64:2<141 2011 64 10725 https://doi.org/10.1007/s10725-010-9548-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9548-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Shijun South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 700 1- Liu Nian College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, 510225, Guangzhou, China aut NATIONALLICENCE 700 1- Sheng Aiwu College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, 510225, Guangzhou, China aut NATIONALLICENCE 700 1- Ma Guohua South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 700 1- Wu Guojiang South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 64/2(2011-06-01), 141-145 0167-6903 64:2<141 2011 64 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer