caa a22 4500 445841192 CHVBK 20180317145344.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10725-010-9516-3 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9516-3 Examining the interaction of light, nutrients and carbohydrates on seed germination and early seedling development of Bletia purpurea (Orchidaceae) [Elektronische Daten] [Timothy Johnson, Michael Kane, Hector Pérez] The effects of carbohydrate availability, carbohydrate source, nutrient availability and illumination on germination and early development of Bletia purpurea (Orchidaceae) seeds were investigated using asymbiotic seed germination. Of special interest was determining the minimum nutritional and light requirements for the completion of germination. Germination and development was limited when seeds were cultured in darkness without sucrose. Seeds were able to germinate under illuminated conditions even in the absence of sucrose and this effect was enhanced when mineral nutrients were incorporated into media. Sucrose, fructose, glucose and trehalose enhanced germination and seedling development while mannitol and sorbitol did not. These data suggest that carbohydrates, either as products of photosynthesis, from symbiotic fungi in situ or as exogenously supplied sugars in vitro, play an important role in regulating seed germination by fulfilling an energy requirement. This hypothesis has been often expressed but rarely satisfactorily tested. Mineral nutrients appear to be less important for germination than carbohydrates. The differential effect of sucrose, fructose, glucose and trehalose at two different concentrations on rhizoid production indicates carbohydrates may play a role in regulating rhizoid production. Springer Science+Business Media B.V., 2010 Nutrition nationallicence Photoblastic nationallicence Rhizoid nationallicence Seed physiology nationallicence Seedling development nationallicence Johnson Timothy Plant Restoration, Conservation & Propagation Biotechnology Laboratory, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut Kane Michael Plant Restoration, Conservation & Propagation Biotechnology Laboratory, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut Pérez Hector Plant Restoration and Conservation Horticulture Consortium, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 89-99 0167-6903 63:1<89 2011 63 10725 https://doi.org/10.1007/s10725-010-9516-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9516-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson Timothy Plant Restoration, Conservation & Propagation Biotechnology Laboratory, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Kane Michael Plant Restoration, Conservation & Propagation Biotechnology Laboratory, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Pérez Hector Plant Restoration and Conservation Horticulture Consortium, Department of Environmental Horticulture, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 89-99 0167-6903 63:1<89 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer