caa a22 4500 445841222 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10725-010-9513-6 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9513-6 Effect of N -( n -butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L [Elektronische Daten] [Ekhiñe Artola, Saioa Cruchaga, Idoia Ariz, Jose Moran, María Garnica, Fabrice Houdusse, José Mina, Ignacio Irigoyen, Berta Lasa, Pedro Aparicio-Tejo] The use of urea as an N fertilizer has increased to such an extent that it is now the most widely used fertilizer in the world. However, N losses as a result of ammonia volatilization lead to a decrease in its efficiency, therefore different methods have been developed over the years to reduce these losses. One of the most recent involves the use of urea combined with urease inhibitors, such as N-(n-butyl) thiophosphoric triamide (NBPT), in an attempt to delay the hydrolysis of urea in the soil. The aim of this study was to perform an in-depth analysis of the effect that NBPT use has on plant growth and N metabolism. Wheat plants were cultivated in a greenhouse experiment lasting 4weeks and fertilized with urea and NBPT at different concentrations (0, 0.012, 0.062, 0.125%). Each treatment was replicated six times. A non-fertilized control was also cultivated. Several parameters related with N metabolism were analysed at the end of growth period. NBPT use was found to have visible effects, such as a transitory yellowing of the leaf tips, at the end of the first week of treatment. At a metabolic level, plants treated with the inhibitor were found to have more urea in their tissues and a lower amino acid content, lower glutamine synthetase activity, and lower urease and glutamine synthetase content at the end of the study period, whereas their urease activity seemed to have recovered by this stage. Springer Science+Business Media B.V., 2010 Ammonium metabolism nationallicence N -( n -butyl) thiophosphoric triamide (NBPT) nationallicence Urea nationallicence Urease nationallicence Urease inhibitor nationallicence Wheat nationallicence Artola Ekhiñe Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Cruchaga Saioa Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Ariz Idoia Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Moran Jose Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Garnica María Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut Houdusse Fabrice Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut Mina José Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut Irigoyen Ignacio Department of Agrarian Production, Public University of Navarre, 31006, Pamplona, Navarre, Spain aut Lasa Berta Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Aparicio-Tejo Pedro Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 73-79 0167-6903 63:1<73 2011 63 10725 https://doi.org/10.1007/s10725-010-9513-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9513-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Artola Ekhiñe Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Cruchaga Saioa Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Ariz Idoia Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Moran Jose Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Garnica María Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut NATIONALLICENCE 700 1- Houdusse Fabrice Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut NATIONALLICENCE 700 1- Mina José Department of R & D, TimacAgro-Grupo Roullier, Polígono Arazuri-Orcoyen, Calle C, No. 32, 31160, Orcoyen, Navarre, Spain aut NATIONALLICENCE 700 1- Irigoyen Ignacio Department of Agrarian Production, Public University of Navarre, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Lasa Berta Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 700 1- Aparicio-Tejo Pedro Institute of Agro-Biotechnology, Public University of Navarre-CSIC-Government of Navarre, Campus de Arrosadía, 31006, Pamplona, Navarre, Spain aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 73-79 0167-6903 63:1<73 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer