caa a22 4500 445841249 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10725-010-9507-4 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9507-4 Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the LEA gene from Tamarix androssowii [Elektronische Daten] [Xin Zhao, Li-Ping Zhan, Xue-Zhong Zou] Previous studies have shown that the late embryogenesis abundant (LEA) gene of Tamarix androssowii can enhance the drought tolerance of transgenic tobacco. In this study, the cloned LEA gene was transformed into half-high bush Northland blueberry in order to enhance its ability to tolerate cold stress. The cephalosporin antibiotics ceftriaxone, cefotaxime and cefazolin were used to optimize transformation of leaf explants, and kanamycin sulfate was used to select for transgenic shoots. PCR and Southern blot analysis confirmed 8 transformants with LEA gene copy numbers ranging from 1 to 7. The LEA chimeric gene was found to be normally transcribed in 6 transgenic lines by RT-PCR. The 8 transgenic lines were tested for cold tolerance by measuring the activities of peroxidase (POD) and superoxide dismutase (SOD), malondialdehyde (MDA) content and relative electrolyte leakage (REL). Overexpression of the LEA gene enhanced the activity of both POD and SOD under low temperature stress conditions. Lipid peroxidation in the transgenic lines was significantly lower than in non-transgenic plants after cold stress, as determined by MDA content and REL. Thus, our findings indicate that the LEA gene confers increased cold tolerance in the Northland blueberry and implicate the metabolic pathways through which it exerts this effect. Springer Science+Business Media B.V., 2010 Northland blueberry nationallicence LEA gene nationallicence Cold tolerance nationallicence Transgenic plant nationallicence Physiological responses nationallicence LEA : Late embryogenesis abundant nationallicence POD : Peroxidase nationallicence SOD : Superoxide dismutase nationallicence MDA : Malondialdehyde nationallicence REL : Relative electrolyte leakage nationallicence ROS : Reactive oxygen species nationallicence Km : Kanamycin nationallicence Zhao Xin College of Forestry, Northeast Forestry University/Key Laboratory of Forestry Genetics and Breeding, 150040, Harbin, Heilongjiang Province, China aut Zhan Li-Ping The Professional and Technological Department of Liaoning University of TCM, 110101, Shenyang, Liaoning Province, China aut Zou Xue-Zhong Liaoning Forestry Vocation-Technical College, 110101, Shenyang, Liaoning Province, China aut Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 13-22 0167-6903 63:1<13 2011 63 10725 https://doi.org/10.1007/s10725-010-9507-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9507-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Xin College of Forestry, Northeast Forestry University/Key Laboratory of Forestry Genetics and Breeding, 150040, Harbin, Heilongjiang Province, China aut NATIONALLICENCE 700 1- Zhan Li-Ping The Professional and Technological Department of Liaoning University of TCM, 110101, Shenyang, Liaoning Province, China aut NATIONALLICENCE 700 1- Zou Xue-Zhong Liaoning Forestry Vocation-Technical College, 110101, Shenyang, Liaoning Province, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 13-22 0167-6903 63:1<13 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer