caa a22 4500 445841265 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10725-010-9488-3 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9488-3 Improving antioxidant activity in transgenic Codonopsis lanceolata plants via overexpression of the γ-tocopherol methyltransferase (γ- tmt) gene [Elektronische Daten] [Bimal Ghimire, Eun Seong, Eun Goh, Eun Kang, Joung Ahn, Chang Yu, Ill Chung] Codonopsis lanceolata Trautv (Companulaceae) is a folk medicine in Korea. To shift the content of tocopherol and enhance its antioxidant properties, we overexpressed the γ-tocopherol methyltransferase (γ-tmt) gene in C. lanceolata. The antioxidant activity of methanolic crude extracts of the transgenic plants was compared to that of control plants using the 1,1-diphenyl-2-picrylhydrazyl radical scavenging method, with α-tocopherol and butylated hydroxy toluene as standard antioxidants. The antioxidant activity of the leaf and root extracts of transgenic plants was higher (IC 50 12-17.33 and 408-524μg/ml, respectively) than that of control plant leaf and root extracts (18 and 529μg/ml, respectively). High-performance liquid chromatography analysis of phenolic compounds confirmed an increase in the levels of 12 major phenolic acids and flavonoids in the leaf and root extracts of transgenic plants compared to control plants. We also found that the rate of photosynthesis was 48% higher in transgenic plants than in control plants. Based on these results, we suggest that increases in the α-tocopherol level in transgenic C. lanceolata plants may result in increases in the photosynthetic performance and antioxidant metabolism of these plants. Springer Science+Business Media B.V., 2010 Antioxidant nationallicence Codonopsis lanceolata nationallicence Transformation nationallicence Transgenic nationallicence α-tocopherol nationallicence γ-tocopherol nationallicence Vitamin E nationallicence Ghimire Bimal Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut Seong Eun Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut Goh Eun Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut Kang Eun Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut Ahn Joung Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut Yu Chang Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut Chung Ill Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 1-6 0167-6903 63:1<1 2011 63 10725 https://doi.org/10.1007/s10725-010-9488-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9488-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghimire Bimal Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Seong Eun Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut NATIONALLICENCE 700 1- Goh Eun Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut NATIONALLICENCE 700 1- Kang Eun Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Ahn Joung Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Yu Chang Bioherb Research Institute, Kangwon National University, 200-701, Chunchon, South Korea aut NATIONALLICENCE 700 1- Chung Ill Department of Applied Life Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 1-6 0167-6903 63:1<1 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer