caa a22 4500 445841273 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10725-010-9508-3 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9508-3 Expression of key antioxidant enzymes under combined effect of heat and cadmium toxicity in growing rice seedlings [Elektronische Daten] [Sareeta Nahakpam, Kavita Shah] Effect of Cd2+ toxicity and heat stress in sensitive rice cv. DR-92 and tolerant rice cv. Bh-1 grown in North East region of India were studied in sand cultures. Increasing levels of 0-500μM Cd2+ alone and/or heat stress showed increased activities of superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase and glutathione reductase enzymes which were associated with induced oxidative stress and altered enzyme activities. The values for SOD and POD activities were always more in cv. DR-92 whereas CAT and GR activities were higher in cv. Bh-1 in roots and shoots under Cd2+ or heat stress alone in sensitive cv. DR-92. Upon imposition of a combination of Cd2++heat the activities of SOD and POD decreased significantly in root/shoot of both the sensitive and tolerant rice varieties. A nine fold increase in GR activity under combination of heat+100μM Cd2+ stress in shoots of cv. Bh-1 at day 15 was noted when compared to controls. The dual stress combination of Cd2++heat did not alter catalase activity in vivo in both the rice varieties. Results suggest a time-specific and varietal distribution of the antioxidant enzymes in rice plants subjected to Cd2+ and/or heat stress. Tolerant cv. Bh-1 has better survival to combined stressors like Cd2+ and heat than sensitive rice cv. DR-92 and heat stress when given in combination with Cd2+ toxicity seem to mitigate the effect of Cd2+ stress alone in rice. The study indicates individual Cd2+ toxicity and heat stress and a combination of the two stresses to have separate implications on antioxidative defense mechanism in rice plants. Among enzymes of the defense apparatus ascorbate peroxidase and glutathione reductase appear to serve as an important component for better survival of rice plants under combination of Cd2++heat stress. Springer Science+Business Media B.V., 2010 Abiotic stress nationallicence Antioxidant enzymes nationallicence Heat shock nationallicence Heavy metal nationallicence Reactive oxygen species nationallicence APX : Ascorbate peroxidase nationallicence CAT : Catalase nationallicence GR : Glutathione reductase nationallicence HSP : Heat shock protein nationallicence MDAR : Monodehydroascorbate reductase nationallicence POD : Peroxidase nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence Nahakpam Sareeta Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Shah Kavita Biochemistry/Bioinformatics Research Lab, Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, 221005, Varanasi, India aut Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 23-35 0167-6903 63:1<23 2011 63 10725 https://doi.org/10.1007/s10725-010-9508-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9508-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nahakpam Sareeta Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Shah Kavita Biochemistry/Bioinformatics Research Lab, Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/1(2011-01-01), 23-35 0167-6903 63:1<23 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer