caa a22 4500 445841303 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9523-4 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9523-4 Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses [Elektronische Daten] [Jin-Huan Chen, Yan Sun, Fang Sun, Xin-Li Xia, Wei-Lun Yin] Ca2+ is a critical component in signal transduction pathways that lead to stress gene expression in higher plants and regulates a wide range of physiological processes. The calcineurin B-like protein (CBL) family represents a unique group of calcium sensors in plants, and plays a key role in decoding calcium transients. Here, a CBL1 homolog gene, AmCBL1 (NCBI accession no. AY902246), was isolated from Ammopiptanthus mongolicus (Maxim.) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Quantitative real-time PCR (QRT-PCR) experiments showed that AmCBL1 was significantly induced by drought, high salinity, heat, and CaCl2 treatments in A. mongolicus seedlings. Subcellular localization analysis suggested that AmCBL1 is a plasma membrane-localized protein. Our results suggest that AmCBL1 is not only a positive regulator of salt response but also a positive regulator of temperature stresses in A. mongolicus. Springer Science+Business Media B.V., 2010 Ammopiptanthus mongolicus nationallicence AmCBL1 nationallicence Abiotic stresses nationallicence Calcium sensor nationallicence ABA : Abscisic acid nationallicence CaM : Calmodulin nationallicence CAML : Calmodulin like proteins nationallicence CBL : Calcineurin B-like nationallicence CDPK : Calcium dependent protein kinase nationallicence CIPK : CBL-interacting protein kinases nationallicence CNB : Calcineurin B nationallicence GFP : Green fluorescent protein nationallicence NCS : Neuronal calcium sensors nationallicence ORF : Open reading frame nationallicence PCR : Polymerase chain reaction nationallicence qRT-PCR : Quantitative real-time PCR nationallicence RACE : Rapid amplification cDNA ends nationallicence Chen Jin-Huan National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut Sun Yan National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut Sun Fang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut Xia Xin-Li National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut Yin Wei-Lun National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 259-269 0167-6903 63:3<259 2011 63 10725 https://doi.org/10.1007/s10725-010-9523-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9523-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jin-Huan National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Sun Yan National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Sun Fang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Xia Xin-Li National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 700 1- Yin Wei-Lun National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 259-269 0167-6903 63:3<259 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer