caa a22 4500 445841311 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9518-1 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9518-1 Production of saponions and polysaccharide in the presence of lactoalbumin hydrolysate in Panax quinquefolium L. cells cultures [Elektronische Daten] [Juan Wang, Wenyuan Gao, Jian Zhang, Tao Huang, Tiantian Wen, Liming Zhang, Luqi Huang] In this article, ginsenosides and polysaccharide contents in suspension cells and native roots of Panax quinquefolium L. were studied. In order to enhance the contents of ginsenosides and polysaccharide in P. quinquefolium suspension cells, we tested the effects of lactoalbumin hydrolysate on the growth of P. quinquefolium suspension cell, synthesis of ginsenosides and polysaccharide in flask and bioreactor. In flask culture, cells growth ratio was significantly enhanced by the addition of lower concentration of lactoalbumin hydrolysate. Addition of 100mgL−1 lactoalbumin hydrolysate significantly enhanced the contents of total saponins (5.44mgg−1 DW) and the contents were 3.89-fold over the control group. Addition of lactoalbumin hydrolysate significantly promoted the accumulation of polysaccharide, except 200mgL−1 lactoalbumin hydrolysate. The highest total saponins yield (36.72mgL−1 DW) and polysaccharide yield (0.83gL−1 DW) were obtained at 100mgL−1 lactoalbumin hydrolysate. In a 5-L stirred tank bioreactor, the highest contents of total saponins and TRb group ginsenosides were achieved on day 26, while the effect of lactoalbumin hydrolysate on the contents of TRg group ginsenosides were insignificant. This result suggests that lactoalbumin hydrolysate might have triggered the enzyme activities for the synthesis of TRb group ginsenosides. Overall, the highest total saponins yield (31.37mgL−1DW) and polysaccharide yield (1.618gL−1 DW) were obtained on day 26 and day 24 respectively and the polysaccharide yield was 1.95-fold higher than the shake flask culture (0.83gL−1 DW). These results provided theoretical reference for two-stage culture in suspension cells of P. quinquefolium in bioreactor. Springer Science+Business Media B.V., 2010 Panax quinquefolium L nationallicence Suspension cell nationallicence Bioreactor nationallicence Elicitor nationallicence Ginsenoside nationallicence Polysaccharide nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence KT : Kinetin nationallicence MS : Murashige and Skoog (1962) nationallicence LH : Lactoalbumin hydrolysate nationallicence TRb : Total Rb group nationallicence TRg : Total Rg group nationallicence Wang Juan School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut Gao Wenyuan School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut Zhang Jian Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Huang Tao School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut Wen Tiantian Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457, Tianjin, China aut Zhang Liming Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457, Tianjin, China aut Huang Luqi Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 217-223 0167-6903 63:3<217 2011 63 10725 https://doi.org/10.1007/s10725-010-9518-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9518-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Juan School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Gao Wenyuan School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Jian Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Huang Tao School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Wen Tiantian Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Liming Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Huang Luqi Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 217-223 0167-6903 63:3<217 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer