caa a22 4500 44584132X CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9521-6 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9521-6 Response of Arabidopsis thaliana roots to lipo-chitooligosaccharide from Bradyrhizobium japonicum and other chitin-like compounds [Elektronische Daten] [Wajahatullah Khan, Carlos Costa, Alfred Souleimanov, Balakrishnan Prithiviraj, Donald Smith] Lipo-chitooligosaccharides (LCOs) are bacteria-to-plant signals required for the establishment of rhizobia-legume nitrogen fixing symbioses. The ability of LCO [Nod Bj V (C18:1, MeFuc)] isolated from B. japonicum (strain 532C), and of oligomers of chitosan (tetramer, pentamer) and chitin (pentamer) to affect the developmental morphology of roots in Arabidopsis thaliana (L.) Heynh ecotype Columbia (Col-0) was assessed using an interactive scanner-based image analysis system. LCOs have been shown to play a role in plant organogenesis at nanomolar concentrations. LCO and the chitin pentamer promoted root growth and development in Arabidopsis at concentrations of 10nM and 100μM, respectively. The LCO treated Arabidopsis plants had about 35% longer roots than untreated control plants. Similarly, treatment with 100μM chitin pentamer (CHIT5) resulted in 26% longer roots than the untreated plants; however, chitosan oligomer (CH4 or CH5) treated plants did not differ from the control plants at either concentration (100 or 1μM). Both LCOs and the chitin pentamer at higher concentrations increased root surface area, mean root diameter and number of root tips. However, leaf area increase was observed only in plants treated with LCO at 10nM. Springer Science+Business Media B.V., 2010 Chitin nationallicence Chitosan nationallicence Lipo-chitooligosaccharides nationallicence Microbial signals nationallicence Root growth nationallicence WinRHIZOTM nationallicence CH4 : Chitosan tetramer nationallicence CH5 : Chitosan pentamer nationallicence CHIT5 : Chitin pentamer nationallicence HPLC : High performance liquid chromatography nationallicence LCO : Lipo-chitooligosaccharides nationallicence Khan Wajahatullah Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia aut Costa Carlos University of Passo Fundo, 99001-970, Passo Fundo, RS, Brazil aut Souleimanov Alfred Department of Plant Science, Macdonald Campus of McGill University, 21111 Lakeshore, Ste., H9X 3V9, Anne-de-Bellevue, QC, Canada aut Prithiviraj Balakrishnan Department of Environmental Sciences, Nova Scotia Agricultural College, B2N 5E3, Truro, NS, Canada aut Smith Donald Department of Plant Science, Macdonald Campus of McGill University, 21111 Lakeshore, Ste., H9X 3V9, Anne-de-Bellevue, QC, Canada aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 243-249 0167-6903 63:3<243 2011 63 10725 https://doi.org/10.1007/s10725-010-9521-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9521-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan Wajahatullah Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Costa Carlos University of Passo Fundo, 99001-970, Passo Fundo, RS, Brazil aut NATIONALLICENCE 700 1- Souleimanov Alfred Department of Plant Science, Macdonald Campus of McGill University, 21111 Lakeshore, Ste., H9X 3V9, Anne-de-Bellevue, QC, Canada aut NATIONALLICENCE 700 1- Prithiviraj Balakrishnan Department of Environmental Sciences, Nova Scotia Agricultural College, B2N 5E3, Truro, NS, Canada aut NATIONALLICENCE 700 1- Smith Donald Department of Plant Science, Macdonald Campus of McGill University, 21111 Lakeshore, Ste., H9X 3V9, Anne-de-Bellevue, QC, Canada aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 243-249 0167-6903 63:3<243 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer