caa a22 4500 445841346 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9530-5 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9530-5 Expression analysis of the cDNA for magnesium chelatase H subunit (CHLH) during sweet cherry fruit ripening and under stress conditions [Elektronische Daten] [Jie Ren, Liang Sun, Canlei Wang, Shengli Zhao, Ping Leng] To understand details about the molecular mechanisms of abscisic acid (ABA) action in fruit maturation, one partial cDNA (PaCHLH1) encoding magnesium chelatase H subunit (CHLH) which was proven to be an ABA receptor in Arabidopsis was cloned from sweet cherry fruit. The sequence of PacCHLH1 was 442bp. The amino acid sequence of PacCHLH1 shows high homology to other CHLHs. PacCHLH1 expression dramatically declined after 25days after full bloom (DAFB) which is the beginning of the ripening process, in contrast to the changes in ABA levels during this period. The application of ABA promoted endogenous ABA biosynthesis and fruit ripening but significantly decreased the expression of PacCHLH1 in fruits. No significant correlation was observed between the PacCHLH1 transcript and ABA accumulation in dehydrated fruits. In dehydrated leaves, the ABA and PacCHLH1 transcript levels were both up-regulated. Springer Science+Business Media B.V., 2010 Sweet cherry nationallicence Abscisic acid nationallicence Magnesium chelatase H subunit (CHLH) nationallicence Receptor nationallicence Ripening nationallicence Ren Jie College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut Sun Liang College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut Wang Canlei College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut Zhao Shengli College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut Leng Ping College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 301-307 0167-6903 63:3<301 2011 63 10725 https://doi.org/10.1007/s10725-010-9530-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9530-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Jie College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Sun Liang College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Wang Canlei College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhao Shengli College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 700 1- Leng Ping College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 301-307 0167-6903 63:3<301 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer