caa a22 4500 445841354 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9528-z doi (NATIONALLICENCE)springer-10.1007/s10725-010-9528-z Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine [Elektronische Daten] [Shengchun Xu, Jin Hu, Yongping Li, Wenguang Ma, Yunye Zheng, Shuijin Zhu] Chilling stress is one of primary constraints to tobacco production in many parts of the world. The present study was conducted to induce chilling tolerance in tobacco by seed priming with putrescine (Put) in relation to physiological changes, using seeds from two tobacco varieties, MSk326 (chilling sensitive variety) and Honghuadajinyuan (HHDJY, chilling tolerant variety). Seed germination, seedling antioxidant enzyme activities and malondialdehyde (MDA) concentration, as well as polyamine concentration were determined under low temperature. During chilling stress at 11°C, seed priming with 0.01mM Put for 48h (Put0.01mM48h) and seed priming with 0.1mM Put for 48h (Put0.1mM48h) significantly increased germination percentage, germination index, seedling length and dry weight of both varieties compared to the controls without Put treatment. When seedlings of 4-leaf stage suffered a short chilling stress (5°C), Put 0.1mM 48h improved the activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), increased endogenous Put, Spd and Spm concentration and decreased the MDA concentration. The results showed that Put priming treatments were available to enhance the chilling tolerance of tobacco seedlings. The optimal treatment of Put was Put0.1mM48h. Springer Science+Business Media B.V., 2010 Antioxidant enzymes nationallicence Chilling nationallicence Nicotiana tabacum L nationallicence Polyamine nationallicence Seedling growth nationallicence Seed priming nationallicence APX : Ascorbate peroxidase nationallicence CAT : Catalase nationallicence EDTA : Ethylenediamine tetra-acetic acid nationallicence MDA : Malondialdehyde nationallicence NBT : Nitroblue tetrazolium nationallicence PA : Polyamine nationallicence POD : Peroxidase nationallicence Put : Putrescine nationallicence SOD : Superoxide dismutase nationallicence Spd : Spermidine nationallicence Spm : Spermine nationallicence TBA : 2-Thiobarbituric acid nationallicence TCA : Trichloroacetic acid nationallicence Xu Shengchun Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut Hu Jin Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut Li Yongping Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut Ma Wenguang Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut Zheng Yunye Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut Zhu Shuijin Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 279-290 0167-6903 63:3<279 2011 63 10725 https://doi.org/10.1007/s10725-010-9528-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9528-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Shengchun Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Jin Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Li Yongping Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut NATIONALLICENCE 700 1- Ma Wenguang Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut NATIONALLICENCE 700 1- Zheng Yunye Southern Center of China Tobacco Breeding Research, Yunnan Tobacco Science Research Institute, 653100, Yuxi, China aut NATIONALLICENCE 700 1- Zhu Shuijin Department of Agronomy, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 279-290 0167-6903 63:3<279 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer