caa a22 4500 445841389 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10725-010-9517-2 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9517-2 MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells [Elektronische Daten] [Jin Wang, Xinrong Li, Yubing Liu, Xin Zhao, Cuiyun Chen, Fang Tian] Programmed cell death (PCD) is an active cellular suicide that occurs both in animals and plants throughout development and in response to abiotic or biotic stress. In contrast to plant hypersensitive response-like cell death, little is known about the molecular machinery that regulates the halophyte plant PCD under high salinity stress. Since mitogen-activated protein kinases (MAPKs) are involved in plant response/tolerance to salt stress, and plant MAPK genes belong to the extracellular signal-regulated kinase (ERK) subfamily, we have investigated the role of ERK-like enzymes in high salinity stress-induced cell death in Thellungiella halophila. The data showed that ERK-like enzymes were early (10min) and transiently activated under 300mM NaCl stress. Pretreatment with 10μM U0126, a special MEK/ERK inhibitor, resulted in a small but statistically significant increase of the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL)-positive nuclei in contrast to salt alone. The effects of U0126 on H2O2 production and cytochrome c (cyt c) release were also investigated. We found that the pretreatment with U0126 accelerated H2O2 production as well as cyt c release, and eventually enhanced cell death. The results suggest that ERK-like enzymes in Thellungiella halophila may act as a positive regulator of salt tolerance, as illustrated by pretreatment with U0126 which enhanced cell death under high salinity stress. Springer Science+Business Media B.V., 2010 Apoptosis nationallicence Cytochrome c nationallicence Hydrogen peroxide nationallicence Mitogen-activated protein kinase nationallicence Salt stress nationallicence Wang Jin Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Li Xinrong Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Liu Yubing Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Zhao Xin Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Chen Cuiyun Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Tian Fang Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 207-216 0167-6903 63:3<207 2011 63 10725 https://doi.org/10.1007/s10725-010-9517-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9517-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jin Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Li Xinrong Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Liu Yubing Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zhao Xin Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Chen Cuiyun Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Tian Fang Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, 730000, Lanzhou, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/3(2011-04-01), 207-216 0167-6903 63:3<207 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer