caa a22 4500 445841427 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10725-010-9555-9 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9555-9 Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9' [Elektronische Daten] [Geert-Jan De Klerk, Huiyuan Guan, Peter Huisman, Svetla Marinova] Stem slices (1-mm thick) cut from apple microshoots were cultured on a modified Murashige-Skoog medium with indole-3-acetic acid (IAA) or α-naphthaleneacetic acid (NAA), and increasing concentrations of various phenolic compounds. Both auxins were added at a concentration suboptimal for rooting. Indole-3-acetic acid is metabolized through oxidation and conjugation but NAA through conjugation only; which might have affected the results. With IAA, all tested orthodiphenols, paradiphenols and triphenols promoted adventitious root formation from the stem slices. Ferulic acid (FA, a methylated orthodiphenol) had the largest effect and increased the number of adventitious roots from 0.9 to 5.8. With NAA there was little or no promotion after addition of phenolics. Phloroglucinol (a triphenol) and FA were examined in detail. Their effects on the dose-response curve of IAA and the timing of their action indicated that both acted as antioxidants protecting IAA from decarboxylation and the tissue from oxidative stress. Experiments with carboxyl-labelled IAA showed that IAA was massively decarboxylated by the slices and that decarboxylation was strongly reduced by phenolics. Decarboxylation was to a great extent attributable to the wound response and did not occur to such an extent in non-wounded plant tissues. In shoots, FA promoted little rooting. Slices were cultured on top of the medium and shoots were stuck into the medium. Possibly, the anaerobic conditions in the medium near the basal part of the stem of shoots reduced the wound response and consequently decarboxylation of IAA. The monophenolic compound salicylic acid (SA) promoted IAA decarboxylation. Accordingly, SA reduced rooting when added during the initial days of the rooting process (the period during which auxin enhances rooting), and promoted outgrowth of root primordia later on (the period during which auxin inhibits rooting). The Author(s), 2011 Rooting nationallicence Oxidative stress nationallicence Ferulic acid nationallicence Phloroglucinol nationallicence Salicylic acid nationallicence FA : Ferulic acid nationallicence IAA : Indole-3-acetic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence PG : Phloroglucinol nationallicence SA : Salicylic acid nationallicence De Klerk Geert-Jan Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut Guan Huiyuan Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut Huisman Peter Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut Marinova Svetla Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 175-185 0167-6903 63:2<175 2011 63 10725 https://doi.org/10.1007/s10725-010-9555-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9555-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Klerk Geert-Jan Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Guan Huiyuan Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Huisman Peter Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Marinova Svetla Wageningen UR Plant Breeding, PO Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 175-185 0167-6903 63:2<175 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer