caa a22 4500 445841435 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10725-011-9562-5 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9562-5 Recalcitrant effects associated with the development of basal callus-like tissue on caulogenesis and rhizogenesis in Sclerocarya birrea [Elektronische Daten] [Mack Moyo, Jeffrey Finnie, Johannes Van Staden] In the micropropagation of woody plant species, adventitious root and shoot formation remain some of the major bottlenecks due to their recalcitrance to in vitro manipulation. Some plant growth regulators may ameliorate these recalcitrant effects and improve in vitro caulogenic and rhizogenic processes. Shoot induction on shoot meristems, hypocotyls and epicotyls was evaluated using equimolar concentrations of benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxytopolin riboside (MemTR). Three auxins, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) were used in the induction of adventitious roots. Moderately high shoot formation (62.7%) was achieved at a concentration of 8.0μM mT after 8weeks in culture. The highest number of adventitious shoots per explant (2.4±0.3) and the longest shoots (23.5±3.16mm) were recorded on 8.0μM mT, though not significantly different from BA treatments. Most shoots progressively produced brown basal callus, which is a potential sink for cytokinin conjugates that are inhibitory to further proliferation of adventitious shoots. Good adventitious shoot formation occurred in 55% of hypocotyl explants on 8.0μM mT. The highest rooting (91.6%) was achieved with IBA-treated shoots at a concentration of 4.0μM. The use of mT and IBA provide an efficient micropropagation method for S. birrea, though further research is required especially in overcoming ex vitro plantlet survival challenges. Springer Science+Business Media B.V., 2011 Aromatic cytokinins nationallicence Auxins nationallicence Caulogenesis nationallicence Recalcitrance nationallicence Rhizogenesis nationallicence BA : 6-Benzyladenine nationallicence IAA : Indole-3-acetic acid nationallicence IBA : Indole-3-butyric acid nationallicence Mem TR : 6-(-3-methoxybenzylamino)-9-β-D-ribofuranosylpurine nationallicence MS : Murashige and Skoog (1962) basal medium nationallicence m T : 6-(3-hydroxybenzylamino)purine nationallicence m TR : 6-(-3-hydroxybenzylamino)-9-β-D-ribofuranosylpurine nationallicence NAA : α-Naphthalene acetic acid nationallicence PVP : Polyvinylpyrrolidone nationallicence Moyo Mack Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Finnie Jeffrey Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 187-195 0167-6903 63:2<187 2011 63 10725 https://doi.org/10.1007/s10725-011-9562-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9562-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moyo Mack Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Finnie Jeffrey Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 187-195 0167-6903 63:2<187 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer