caa a22 4500 445841451 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10725-010-9558-6 doi (NATIONALLICENCE)springer-10.1007/s10725-010-9558-6 Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments [Elektronische Daten] [Michael Bairu, Ondřej Novák, Karel Doležal, Johannes Van Staden] Changes in cytokinin (CK) profiles and their physiological implications in micropropagated Harpagophytum procumbens [(Burch.) DC. ex Meisn.] tissues in relation to shoot-tip necrosis (STN) and CK treatments were studied. Total CK content was quantified in benzyladenine (BA)-treated necrotic and normal plantlets and in plantlets treated with the CKs BA, meta-topolin (mT) and meta-topolin riboside (mTR) with and without the auxin indole-3-acetic acid (IAA). Generally necrotic shoots yielded more total CK compared to normal shoots. Cytokinin accumulation was higher at the basal section (basal>middle>top). Further analysis of the CKs based on structural and functional forms revealed excessive accumulation of 9-glucosides (deactivation products—toxic metabolites) and limited amounts of O-glucosides (storage forms—re-utilizable) in necrotic and BA-treated shoots compared to normal and topolin-treated cultures. The addition of IAA enhanced the formation of 9-glucosides in BA-treated cultures but reduced it in topolin-treated cultures. The symptom of STN could therefore be attributed to conversion of active cytokinins to other forms such as 9-glucosides which are neither active nor reversibly sequestrated to active forms. Literature shows that metabolites like 9-glucosides of BA have a detrimental effect in plant tissue culture. Springer Science+Business Media B.V., 2010 Auxin cytokinin interaction nationallicence Cytokinin purification and analysis nationallicence Devil's claw nationallicence Glucosylation nationallicence Harpagophytum procumbens nationallicence Micropropagation nationallicence Pedaliaceae nationallicence Shoot-tip necrosis nationallicence BA : N6-benzyladenine nationallicence BA9G : N6-benzyladenine-9-glucoside nationallicence BAR : N6-benzyladenosine nationallicence BAR5′MP : N6-benzyladenosine-5′-monophosphate nationallicence CK : Cytokinin nationallicence cZ : cis-zeatin nationallicence cZ9G : cis-zeatin-9-glucoside nationallicence cZOG : cis-zeatin-O-glucoside nationallicence cZR : cis-zeatin riboside nationallicence cZR5′MP : cis-zeatin riboside-5′-monophosphate nationallicence cZROG : cis-zeatin-O-glucoside riboside nationallicence DHZ : Dihydrozeatin nationallicence DHZ9G : Dihydrozeatin-9-glucoside nationallicence DHZOG : Dihydrozeatin-O-glucoside nationallicence DHZR : Dihydrozeatin riboside nationallicence DHZR5′MP : Dihydrozeatin riboside-5′-monophosphate nationallicence DHZROG : Dihydrozeatin-O-glucoside riboside nationallicence iP : N6-isopentenyladenine nationallicence iP9G : N6-isopentenyladenine-9-glucoside nationallicence iPR : N6-isopentenyladenosine nationallicence iPR5′MP : N6-isopentenyladenosine-5′-monophosphate nationallicence IAC : Immunoaffinity chromatography nationallicence MRM : Multiple reaction monitoring nationallicence MS medium : Murashige and Skoog (1962) basal medium nationallicence m T : meta-topolin nationallicence m T9G : meta-topolin-9-glucoside nationallicence m TOG : meta-topolin-O-glucoside nationallicence m TR : meta-topolin riboside nationallicence m TR5′MP : meta-topolin-5′-monophosphate nationallicence m TROG : meta-topolin-O-glucoside riboside nationallicence o T : ortho-topolin nationallicence o T9G : ortho-topolin-9-glucoside nationallicence o TOG : ortho-topolin-O-glucoside nationallicence o TR : ortho-topolin riboside nationallicence o TR5′MP : ortho-topolin-5′-monophosphate nationallicence o TROG : ortho-topolin-O-glucoside riboside nationallicence p T : para-topolin nationallicence p TOG : para-topolin-O-glucoside nationallicence p TR : para-topolin riboside nationallicence p TR5′MP : para-topolin-5′-monophosphate nationallicence p TROG : para-topolin-O-glucoside riboside nationallicence STN : Shoot-tip necrosis nationallicence tZ : trans-zeatin nationallicence tZ9G : trans-zeatin-9-glucoside nationallicence tZOG : trans-zeatin-O-glucoside nationallicence tZR : trans-zeatin riboside nationallicence tZR5′MP : trans-zeatin riboside-5′-monophosphate nationallicence tZROG : trans-zeatin-O-glucoside riboside nationallicence UPLC : Ultra performance liquid chromatography nationallicence Bairu Michael Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Novák Ondřej Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany AS CR, Šlechtitelů 11, 783 71, Olomouc, Czech Republic aut Doležal Karel Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany AS CR, Šlechtitelů 11, 783 71, Olomouc, Czech Republic aut Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 105-114 0167-6903 63:2<105 2011 63 10725 https://doi.org/10.1007/s10725-010-9558-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-010-9558-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bairu Michael Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 700 1- Novák Ondřej Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany AS CR, Šlechtitelů 11, 783 71, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Doležal Karel Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany AS CR, Šlechtitelů 11, 783 71, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 63/2(2011-03-01), 105-114 0167-6903 63:2<105 2011 63 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer