caa a22 4500 445841494 CHVBK 20180317145345.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9576-z doi (NATIONALLICENCE)springer-10.1007/s10725-011-9576-z A novel Phytochrome B allele in Arabidopsis thaliana exhibits partial mutant phenotype: a short deletion in N-terminal extension reduces Phytochrome B activity [Elektronische Daten] [Scott Nicholson, Ute Hoecker, Vibha Srivastava] During analysis of an Arabidopsis thaliana line possessing a Phytochrome A epiallele (phyA'), a partial Phytochrome B-deficient phenotype was observed, consisting of elongated hypocotyls in seedlings grown under constant white light or red light (660nm). The observed hypocotyls were twice the length (8mm) of wild-type (4mm), but approximately half the length of a null phyB-9 mutant (14mm). Several analyses were performed to characterize this apparent partial phyB mutant. Sequencing of the entire exonic region revealed three point mutations that altered codon usage, and one in-frame 12base pair deletion. Each of the point mutations has been described in other lines that display wild-type phenotype, and therefore their effect is thought to be minimal, if any. The N-terminal deletion of amino acids 9 through 12 (GGGR) is a unique mutation found in this line. This deletion most likely contributes to the phyB mutant phenotype by lowering the binding affinity of the active form of Phytochrome B (Pfr) with Phytochrome Interacting Factor 3 (PIF3). Springer Science+Business Media B.V., 2011 phyA nationallicence phyB nationallicence Epiallele nationallicence Hypocotyl length nationallicence PIF nationallicence Nicholson Scott Department of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA aut Hoecker Ute Botanical Institute, University of Cologne, Zülpicher Str. 47b, 50674, Koeln, Germany aut Srivastava Vibha Department of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 207-212 0167-6903 65:1<207 2011 65 10725 https://doi.org/10.1007/s10725-011-9576-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9576-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nicholson Scott Department of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA aut NATIONALLICENCE 700 1- Hoecker Ute Botanical Institute, University of Cologne, Zülpicher Str. 47b, 50674, Koeln, Germany aut NATIONALLICENCE 700 1- Srivastava Vibha Department of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 207-212 0167-6903 65:1<207 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer