caa a22 4500 445841532 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9569-y doi (NATIONALLICENCE)springer-10.1007/s10725-011-9569-y Suppression and overexpression of Lsi1 induce differential gene expression in rice under ultraviolet radiation [Elektronische Daten] [Chang-Xun Fang, Qing-Shui Wang, Yan Yu, Qing-Mao Li, Hai-Li Zhang, Xing-Chun Wu, Ting Chen, Wen-Xiong Lin] Low silicon rice gene 1 (Lsi1) belongs to a member of Nod26-like major intrinsic protein (NIP) subfamily and is thought to control silicon (Si) accumulation in rice. In order to further elucidate its regulatory mechanisms in the defense of rice plants to enhanced ultraviolet-B (UV-B) radiation stress, Lsi1 was subjected to suppressed and overexpressed treatments in a UV-B tolerance rice accession Lemont as well as to overexpressed treatment in a UV-B sensitive rice accession Dular. The results showed that transcript levels of Lsi1 increased in Lsi1-overexpressed transgenic lines of rice accessions Lemont and Dular, but down-regulated in Lsi1-RNAi transgenic line of Lemont in comparison with their wild types (WT). A similar tendency was found in the root Si uptake and the Si concentrations in leaves. Further, the different transgenic rice lines and their WT were exposed to enhance UV-B radiation, study by suppression subtractive hybridization (SSH) found that Lsi1 not only could regulate the expression of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL) and photolyase (PL), but also could induce other signal transduction-, detoxification-, resistance-, and photosynthesis-related genes. The findings suggested that the regulation of silicon nutrient by enhancing/inhibiting expression of Lsi1 could effectively induce the transcription of the mRNAs relative to tolerance in rice. Springer Science+Business Media B.V., 2011 Lsi1 nationallicence Rice ( Oryza sativa L.) nationallicence Silicon nationallicence UV-B radiation nationallicence 4CL : 4-coumarate-CoA ligase nationallicence Lsi1 : Low silicon rice 1 nationallicence PL : Photolyase nationallicence PAL : Phenylalanine ammonia-lyase nationallicence Si : Silicon nationallicence SSH : Suppression subtractive hybridization nationallicence UV-B : Ultraviolet-B nationallicence WT : Wild types nationallicence Fang Chang-Xun Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Wang Qing-Shui Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Yu Yan Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Li Qing-Mao Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Zhang Hai-Li Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Wu Xing-Chun Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Chen Ting Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Lin Wen-Xiong Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 1-10 0167-6903 65:1<1 2011 65 10725 https://doi.org/10.1007/s10725-011-9569-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9569-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fang Chang-Xun Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qing-Shui Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Yan Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Qing-Mao Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hai-Li Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xing-Chun Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Ting Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lin Wen-Xiong Institute of Agroecology/School of Life Sciences, Fujian Agriculture and Forestry University (FAFU), 350002, Fuzhou, People's Republic of China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 1-10 0167-6903 65:1<1 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer