caa a22 4500 445841540 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9580-3 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9580-3 Overexpression of a maize dehydrin gene, ZmDHN2b , in tobacco enhances tolerance to low temperature [Elektronische Daten] [Xin Xing, Yukun Liu, Xiangpei Kong, Yang Liu, Dequan Li] Dehydrins, a subfamily of group 2 LEA proteins, are intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. In this study, we isolated and characterized ZmDHN2b, a maize dehydrin gene. The genomic organization of the ZmDHN2b gene and its expression in maize seedlings were analyzed. To investigate the function of ZmDHN2b, we generated transgenic tobacco plants constitutively overexpressing ZmDHN2b. Ectopic expression of ZmDHN2b in tobacco accelerated seed germination and seedling growth at 15°C. Furthermore, ZmDHN2b-overexpressing lines had lower levels of cold-induced malondialdehyde and less electrolyte leakage than wild-type tobacco at 4°C. These results demonstrated that ZmDHN2b was involved in plant responses to low temperature. Springer Science+Business Media B.V., 2011 Maize nationallicence Dehydrin nationallicence ZmDHN2b nationallicence Low temperature nationallicence Transgenic tobacco nationallicence ABA : Abscisic acid nationallicence DHN : Dehydrin nationallicence GA : Gibberellic acid nationallicence H2O2 : Hydrogen peroxide nationallicence JA : Jasmonic acid nationallicence LEA : Late embryogenesis abundant nationallicence ORF : Open reading frame nationallicence PCR : Polymerase chain reaction nationallicence pI : Isoelectric point nationallicence SA : Salicylic acid nationallicence SDS-PAGE : Sodium dodecyl sulfate-polyacrylamide gel electrophoresis nationallicence TBA : Thiobarbituric acid nationallicence TCA : Trichloroacetic acid nationallicence WT : Wild type nationallicence Xing Xin State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut Liu Yukun State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut Kong Xiangpei State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut Liu Yang State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut Li Dequan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 109-118 0167-6903 65:1<109 2011 65 10725 https://doi.org/10.1007/s10725-011-9580-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9580-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xing Xin State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut NATIONALLICENCE 700 1- Liu Yukun State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut NATIONALLICENCE 700 1- Kong Xiangpei State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut NATIONALLICENCE 700 1- Liu Yang State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut NATIONALLICENCE 700 1- Li Dequan State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 DaiZong Street, 271018, Tai'an, Shandong, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 109-118 0167-6903 65:1<109 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer