caa a22 4500 445841559 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9575-0 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9575-0 Identification and expression analysis of genes induced by phosphate starvation in leaves and roots of Brassica napus [Elektronische Daten] [Feng Ren, Liang Chen, Qian-Qian Guo, Hui Zhong, Yan Wu, Li-Li Chang, Xue-Bao Li] Phosphorus (P) is one of the essential nutrient elements for plant growth and development because of its low availability and mobility in soil. To investigate genes which are involved in Pi starvation response in leaves and roots of Brassica napus, a survey of genes induced in Pi starvation was done by macroarray analysis. Among 3,000 cDNA clones, 94 genes were identified to be up-regulated, while 46 genes were detected to be down-regulated by Pi starvation in B. napus. The Pi starvation-induced genes represent a large range of functional categories including cell defense, cellular signal transduction, cellular transport, biogenesis of cellular components, protein synthesis, proteins with binding function, regulation of metabolism and so on. By comparative analysis, over 40% genes were overlapped with Pi starvation-induced genes in Arabidopsis, but the remaining was not identified in Arabidopsis. From the genes identified by macroarray analysis, 22 genes were selected for further quantitative reverse transcription (RT)-PCR assay and confirmed as Pi starvation-induced genes convincingly. The temporal expression patterns of 22 genes in roots and leaves were different during Pi starvation. Most of the 22 genes were expressed at relatively low level in leaves and roots, and at relatively high level in other tissues. The data presented in this study will facilitate the understanding of molecular mechanism of Pi starvation response in B. napus. Springer Science+Business Media B.V., 2011 Brassica napus nationallicence Macroarray nationallicence Phosphate starvation nationallicence Gene identification nationallicence Ren Feng Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Chen Liang Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Guo Qian-Qian Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Zhong Hui Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Wu Yan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Chang Li-Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Li Xue-Bao Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 65-81 0167-6903 65:1<65 2011 65 10725 https://doi.org/10.1007/s10725-011-9575-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9575-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Feng Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Chen Liang Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Guo Qian-Qian Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Zhong Hui Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Wu Yan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Chang Li-Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Li Xue-Bao Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, 430079, Wuhan, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 65-81 0167-6903 65:1<65 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer