caa a22 4500 445841567 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9573-2 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9573-2 Production of psoralen by in vitro regenerated plants from callus cultures of Psoralea corylifolia L [Elektronische Daten] [P. Baskaran, N. Jayabalan, J. Van Staden] Psoralea corylifolia is an attractive, endangered annual producing various bioactive compounds of medical importance. This plant contributes to Indian pharmaceutical and cosmetic industries for the production of commercial medicines, Ayurvedic skin care ointments and soap. The influence of various plant growth regulators (PGRs) and additives on high-frequency rapid adventitious shoot regeneration from transverse thin cell layer (tTCL) hypocotyl explants of P. corylifolia was investigated. Organogenic callus was obtained in tTCL hypocotyl explants on Murashige and Skoog (1962) medium supplemented with 15μM naphthaleneacetic acid (NAA) and 3μM benzylaminopurine (BA). The highest adventitious shoot regeneration (107.5 shoots per explant) was achieved in culture when transferred to half-strength solid MS medium. The regenerated shoots were rooted and the plantlets successfully acclimatized in moistened (1/8-MS basal salt solution with 3μM indole-3-butyric acid (IBA), 1μM 2-isopentenyladenine (2iP) and 100mgl−1 Bavistin (BVN)); garden soil, farmyard soil and sand (2:1:1, v/v/v). The acclimatized plants produced flowers in the growth chamber. When planted in the field these plants set viable seed. The psoralen content in different tissues of ex vitro and naturally-grown plants was determined by high-performance liquid chromatography (HPLC). The highest psoralen content was recorded in seeds from naturally-grown (6.48μgg−1DW) and ex vitro plants (6.46μgg−1DW). This system can be used for rapid mass propagation of P. corylifolia, for conservation strategies, and to produce phytomedicines. Springer Science+Business Media B.V., 2011 Callus cultures nationallicence In vitro regeneration nationallicence Psoralea corylifolia nationallicence Psoralen nationallicence Transverse thin cell layers nationallicence Ads : Adenine sulphate nationallicence BA : N6-benzylaminopurine nationallicence BVN : Bavistin nationallicence CH : Casein hydrolysate nationallicence CW : Coconut water nationallicence HPLC : High-performance liquid chromatography nationallicence IBA : Indole-3-butyric acid nationallicence 2iP : 2-isopentenyladenine nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog nationallicence NAA : α-Naphthaleneacetic acid nationallicence PGRs : Plant growth regulators nationallicence tTCLs : Transverse thin cell layers nationallicence Baskaran P. Department of Plant Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut Jayabalan N. Department of Plant Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut Van Staden J. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, 3209, Pietermaritzburg, Scottsville, South Africa aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 47-54 0167-6903 65:1<47 2011 65 10725 https://doi.org/10.1007/s10725-011-9573-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9573-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baskaran P. Department of Plant Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut NATIONALLICENCE 700 1- Jayabalan N. Department of Plant Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, Tamil Nadu, India aut NATIONALLICENCE 700 1- Van Staden J. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, 3209, Pietermaritzburg, Scottsville, South Africa aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 47-54 0167-6903 65:1<47 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer