caa a22 4500 445841575 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9577-y doi (NATIONALLICENCE)springer-10.1007/s10725-011-9577-y Heat stress screening of peanut ( Arachis hypogaea L.) seedlings for acquired thermotolerance [Elektronische Daten] [Michael Selvaraj, Gloria Burow, John Burke, Vikas Belamkar, Naveen Puppala, Mark Burow] Heat can be one of the major abiotic stresses that adversely affect crop production worldwide at different stages of development. As field screening for heat tolerance can be inconsistent and seasonally-limited, it is important to develop a reliable protocol under controlled conditions that allows simultaneous screening of multiple genotypes. The objective of this research was to develop a straightforward laboratory protocol using acquired thermotolerance (ATT) in peanut seedlings as a measure of one mechanism of heat stress tolerance. Sixteen genotypes, including selected accessions of the US peanut minicore collection along with standard checks, were evaluated for acquired themotolerance in two independent experiments. A change in the temperature sensitivity of chlorophyll accumulation was used as an indicator of acquired thermotolerance. Pre-incubation at 38°C for 4h before the 30-min 50°C challenge triggered the acquired thermotolerance system of the leaf disks, resulting in chlorophyll accumulation upon exposure to light. There was considerable variation among genotypes for ATT in both experiments. Genotypic ranking for mean ATT values were highly correlated (0.949) in both experiments. The effect of seed weight on ATT was not significant. This method is relatively simple and inexpensive and can be used to screen a large number of genotypes. Springer Science+Business Media B.V., 2011 Acquired thermotolerance nationallicence Bioassay nationallicence Cellular thermo electric controller nationallicence Peanut nationallicence US peanut minicore nationallicence Selvaraj Michael Texas AgriLife Research, Texas A&M System, 1102 East FM 1294, 79403, Lubbock, TX, USA aut Burow Gloria USDA-ARS Plant Stress and Germplasm Development Unit, 3810 4th Street, 79415, Lubbock, TX, USA aut Burke John USDA-ARS Plant Stress and Germplasm Development Unit, 3810 4th Street, 79415, Lubbock, TX, USA aut Belamkar Vikas Department of Plant and Soil Science, Texas Tech University, 79409, Lubbock, TX, USA aut Puppala Naveen Agricultural Sciences Center, New Mexico State University, 88001, Clovis, NM, USA aut Burow Mark Texas AgriLife Research, Texas A&M System, 1102 East FM 1294, 79403, Lubbock, TX, USA aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 83-91 0167-6903 65:1<83 2011 65 10725 https://doi.org/10.1007/s10725-011-9577-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9577-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Selvaraj Michael Texas AgriLife Research, Texas A&M System, 1102 East FM 1294, 79403, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Burow Gloria USDA-ARS Plant Stress and Germplasm Development Unit, 3810 4th Street, 79415, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Burke John USDA-ARS Plant Stress and Germplasm Development Unit, 3810 4th Street, 79415, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Belamkar Vikas Department of Plant and Soil Science, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Puppala Naveen Agricultural Sciences Center, New Mexico State University, 88001, Clovis, NM, USA aut NATIONALLICENCE 700 1- Burow Mark Texas AgriLife Research, Texas A&M System, 1102 East FM 1294, 79403, Lubbock, TX, USA aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 83-91 0167-6903 65:1<83 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer