caa a22 4500 445841583 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9581-2 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9581-2 Study of the antioxidant enzymatic system during shoot development from cultured intercalar meristems of saffron [Elektronische Daten] [Pedro Díaz-Vivancos, Khalid Majourhat, José Fernández, José Hernández, Abel Piqueras] As preliminary research to develop a clonal in vitro propagation system for saffron, we have evaluated the morphogenic response of intercalary meristems present in shoots from sprouting saffron corms when cultured in media with different cytokinins. The best results in terms of shoot production and quality were achieved in the medium supplemented with 5mg/l of benzylaminopurine (BAP). During the multiplication of the meristems, several changes in the activities of the antioxidant enzymatic system were detected. Catalase (CAT) activity was reduced in the presence of the assayed cytokinins, whereas peroxidase (POX) activity was stimulated with all the cytokinins assayed. When TDZ (thidizuron) was used, a slight decrease in superoxide dismutase (SOD) activity occurred, whereas BAP and 2iP (2-isopentenyl adenine) treatments produced a significant increase in SOD activity. The ascorbate-glutathione cycle enzymes showed a similar pattern in response to the addition of cytokinins. These results suggest a possible use of antioxidant enzyme activities as a parameter that could be use to evaluate the efficiency of micropropagation protocols for saffron and even in other species. Springer Science+Business Media B.V., 2011 Antioxidants enzymes nationallicence Cytokinins nationallicence BAP nationallicence Crocus sativus nationallicence Intercalar meristems nationallicence Micropropagation nationallicence 2iP : 2-isopentyladenin nationallicence APX : Ascorbate peroxidase (EC 1.11.1.1) nationallicence BAP : 6-benzylaminopurine nationallicence CAT : Catalase (EC 1.11.1.6) nationallicence DHAR : Dehydroascorbate reductase (EC 1.8.5.1) nationallicence GR : Glutathione reductase (EC 1.6.4.2) nationallicence MDHAR : Monodehydroascorbate reductase (EC 1.6.5.4) nationallicence POX : Peroxidases (EC 1.11.1.7) nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase (EC 1.15.1.1) nationallicence TDZ : Thidiazuron nationallicence Díaz-Vivancos Pedro Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut Majourhat Khalid Faculté des Sciences Semlalia, University Cadi-Ayyad of Marrakech, BP 2390, 40000, Marrakech, Morocco aut Fernández José Department of Biotechnology, IDR-University of Castilla-La Mancha, Campus Universitario de Albacete s/n, 02071, Albacete, Spain aut Hernández José Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut Piqueras Abel Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 119-126 0167-6903 65:1<119 2011 65 10725 https://doi.org/10.1007/s10725-011-9581-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9581-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Díaz-Vivancos Pedro Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Majourhat Khalid Faculté des Sciences Semlalia, University Cadi-Ayyad of Marrakech, BP 2390, 40000, Marrakech, Morocco aut NATIONALLICENCE 700 1- Fernández José Department of Biotechnology, IDR-University of Castilla-La Mancha, Campus Universitario de Albacete s/n, 02071, Albacete, Spain aut NATIONALLICENCE 700 1- Hernández José Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Piqueras Abel Department of Plant Breeding, CEBAS-CSIC, PO Box 164, 30100, Espinardo, Murcia, Spain aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 119-126 0167-6903 65:1<119 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer