caa a22 4500 44584163X CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9578-x doi (NATIONALLICENCE)springer-10.1007/s10725-011-9578-x Differential expression of farnesyl diphosphate synthase gene from Withania somnifera in different chemotypes and in response to elicitors [Elektronische Daten] [Parul Gupta, Nehal Akhtar, Shri Tewari, Rajender Sangwan, Prabodh Trivedi] Withania somnifera (L.) Dunal (Family, Solanaceae), commonly known as Ashwagandha is one of the most valuable medicinal plants synthesizing large number of pharmacologically active secondary metabolites known as withanolides. Though the plant has been well characterized in terms of phytochemical profiles as well as pharmaceutical activities, not much is known about the genes responsible for biosynthesis of these compounds. In this study, we have characterized a gene encoding farnesyl diphosphate synthase (FPPS; EC 2.5.1.10), a key enzyme in the pathway of biosynthesis of isoprenoids, from W. somnifera. The full-length cDNA of Withania somnifera FPPS (WsFPPS) of 1,253bps encodes a polypeptide of 343 amino acids. The amino acid sequence homology and phylogenetic analysis suggest that WsFPPS has close similarity to its counterparts from tomato (SlFPPS) and capsicum (CaaFPPS). Using semi quantitative RT-PCR, the expression pattern of the WsFPPS gene was analyzed in different tissues of Withania chemotypes (NMITLI-101, NMITLI-108, NMITLI-118 and NMITLI-135) as well as in response to elicitors (salicylic acid and methyl jasmonate) and mechanical wounding. The expression analysis suggests that WsFPPS expression varies in different tissues (with maximal expression in flower and young leaf) and chemotypes (with highest level in NMITLI-101) and was significantly elevated in response to salicylic acid, methyl jasmonate and mechanical injury. This is the first report on characterization of an isoprenoid pathway gene involved in withanolide biosynthesis. Springer Science+Business Media B.V., 2011 Elicitor response nationallicence Medicinal plant nationallicence Mevalonate pathway nationallicence Withanolides nationallicence WsFPPS nationallicence Gupta Parul National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut Akhtar Nehal National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut Tewari Shri National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut Sangwan Rajender Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, 226015, Lucknow, India aut Trivedi Prabodh National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 93-100 0167-6903 65:1<93 2011 65 10725 https://doi.org/10.1007/s10725-011-9578-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9578-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gupta Parul National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut NATIONALLICENCE 700 1- Akhtar Nehal National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut NATIONALLICENCE 700 1- Tewari Shri National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut NATIONALLICENCE 700 1- Sangwan Rajender Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, 226015, Lucknow, India aut NATIONALLICENCE 700 1- Trivedi Prabodh National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, 226 001, Lucknow, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 93-100 0167-6903 65:1<93 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer