caa a22 4500 445841648 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9587-9 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9587-9 Response of in vitro strawberry to antibiotics [Elektronische Daten] [Y. Qin, Jaime Teixeira da Silva, J. Bi, S. Zhang, G. Hu] By identifying antibiotics that had the least phytotoxic effects on explants during genetic transformation, we evaluated the effect of various antibiotics on callus induction and morphogenesis from leaf explants and in vitro growth of Fragaria×ananassa Duch. cv. Toyonaka. Results showed that kanamycin (Kan) significantly inhibited callus induction, bud differentiation and root morphogenesis while carbenicillin (Carb), cefotaxime (Cef) and an equal concentration of Cef and Carb up to 500mgL−1 had no significant effects on callus induction and shoot growth. Kan, even at 2.5mgL−1, significantly inhibited callus induction, shoot regeneration and root formation, while no shoots regenerated at concentrations above 15mgL−1. Rooting was completely inhibited in the presence of 50mgL−1 Kan. Cef had negative effects on shoot regeneration from leaf explants and in vitro growth of strawberry. Compared to Cef, Carb at≤300mgL−1 significantly promoted shoot and root organogenesis. However, an equal concentration of Carb plus Cef could alleviate the negative effect of Cef on strawberry. Results from relative electrolyte leakage, root and antioxidant activities, O2·− production rate, H2O2, proline and MDA contents showed that Kan, Cef and Carb caused electrolyte leakage and triggered active enzymatic processes and metabolism. This offers a possible mechanism for the inhibition or stimulation of strawberry growth caused by these antibiotics. Springer Science+Business Media B.V., 2011 Fragaria × ananassa Duch nationallicence Antibiotics nationallicence Shoot regeneration nationallicence Antioxidant activities nationallicence Qin Y. College of Horticulture, South China Agricultural University, 510642, Guangzhou, China aut Teixeira da Silva Jaime Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, 761-0795, Ikenobe, Kagawa, Japan aut Bi J. Department of Horticulture, Zhejiang University, 310029, Hangzhou, China aut Zhang S. Department of Horticulture, Zhejiang University, 310029, Hangzhou, China aut Hu G. College of Horticulture, South China Agricultural University, 510642, Guangzhou, China aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 183-193 0167-6903 65:1<183 2011 65 10725 https://doi.org/10.1007/s10725-011-9587-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9587-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Y. College of Horticulture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Teixeira da Silva Jaime Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, 761-0795, Ikenobe, Kagawa, Japan aut NATIONALLICENCE 700 1- Bi J. Department of Horticulture, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang S. Department of Horticulture, Zhejiang University, 310029, Hangzhou, China aut NATIONALLICENCE 700 1- Hu G. College of Horticulture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 183-193 0167-6903 65:1<183 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer