caa a22 4500 445841672 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10725-011-9572-3 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9572-3 Role of proline and glycinebetaine pretreatments in improving heat tolerance of sprouting sugarcane ( Saccharum sp.) buds [Elektronische Daten] [Rizwan Rasheed, A. Wahid, M. Farooq, Iqbal Hussain, Shahzad Basra] High temperature strongly hampers the plant growth particularly at early growth stages. In this study, changes in some physiological and anatomical characteristics and possibility of mitigating the adversities of heat stress by soaking sugarcane nodal buds in 20mM proline and glycinebetaine (GB) solutions have been explored. Heat stress reduced the rate of bud sprouting nonetheless soaking the setts in proline followed by GB was beneficial. In addition, heat stress reduced the bud fresh and dry weights, generated H2O2, reduced the tissue levels of K+ and Ca2+, while increased the osmolytes synthesis in a time course manner. Heat stress also delayed the emergence and expansion of new bud leaves, by restricting the number and area of mesophyll cells. It also caused poor and aberrant development and diffused appearance of mesophyll cells and vascular bundles in the bud leaves. However, soaking of buds in proline and GB solutions substantially reduced the H2O2 production, improved the accumulation of soluble sugars and protected the developing tissues from heat stress effects; although proline was more effective than GB. Correlations of various attributes indicated that soaking in GB and proline restricted the H2O2 generation, improved K+ and Ca2+ contents, and increased the concentrations of free proline, GB and soluble sugars eventually improving the heat tolerance of buds. Cost-benefit analysis showed that, considering increase in sprouting of buds, soaking in 20mM solution of both osmoprotectants is economical. Springer Science+Business Media B.V., 2011 Bud sprouting nationallicence Glycinebetaine nationallicence Heat stress nationallicence Mesophyll cells nationallicence Nutrients nationallicence Sugarcane nationallicence Rasheed Rizwan Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut Wahid A. Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut Farooq M. Department of Agronomy, University of Agriculture, 38040, Faisalabad, Pakistan aut Hussain Iqbal Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut Basra Shahzad Department of Crop Physiology, University of Agriculture, 38040, Faisalabad, Pakistan aut Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 35-45 0167-6903 65:1<35 2011 65 10725 https://doi.org/10.1007/s10725-011-9572-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9572-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rasheed Rizwan Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Wahid A. Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Farooq M. Department of Agronomy, University of Agriculture, 38040, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Hussain Iqbal Department of Botany, University of Agriculture, 38040, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Basra Shahzad Department of Crop Physiology, University of Agriculture, 38040, Faisalabad, Pakistan aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/1(2011-09-01), 35-45 0167-6903 65:1<35 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer