caa a22 4500 445841729 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10725-011-9622-x doi (NATIONALLICENCE)springer-10.1007/s10725-011-9622-x New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical [Elektronische Daten] [Budi Winarto, F. Rachmawati, Jaime Teixeira da Silva] A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0mg/l), α-naphthalene acetic acid (0.01-0.2mg/l), thidiazuron (0.5-2.0mg/l), 6-benzylaminopurine (0.5-1.0mg/l), and kinetin (0.5-1.0mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01mg/l α-naphthalene acetic acid, 0.5mg/l thidiazuron and 1.0mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25mg/l 2,4-dichlorophenoxy acetic acid, 0.02mg/l α-naphthalene acetic acid, 1.5mg/l thidiazuron and 0.75mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2mg/l α-naphthalene acetic acid and 1.0mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n=14-18), 15 aneuploid (n=20-26), 126 diploid (n=28-34) and 5 triploid (n=45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed. Springer Science+Business Media B.V., 2011 Basal medium nationallicence Anther culture nationallicence Cytology nationallicence Anthurium andreanum nationallicence Linden ex André cv. Tropical nationallicence Tissue culture nationallicence Acclimatization nationallicence Winarto Budi Tissue Culture Laboratory, Indonesian Ornamental Crops Research Institute, Sindanglaya, P.O. Box 8, 43253, Pacet-Cianjur, West Java, Indonesia aut Rachmawati F. Tissue Culture Laboratory, Indonesian Ornamental Crops Research Institute, Sindanglaya, P.O. Box 8, 43253, Pacet-Cianjur, West Java, Indonesia aut Teixeira da Silva Jaime Faculty of Agriculture and Graduate School, Kagawa University, Miki-cho, 761-0795, Kagawa, Japan aut Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 513-529 0167-6903 65:3<513 2011 65 10725 https://doi.org/10.1007/s10725-011-9622-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9622-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Winarto Budi Tissue Culture Laboratory, Indonesian Ornamental Crops Research Institute, Sindanglaya, P.O. Box 8, 43253, Pacet-Cianjur, West Java, Indonesia aut NATIONALLICENCE 700 1- Rachmawati F. Tissue Culture Laboratory, Indonesian Ornamental Crops Research Institute, Sindanglaya, P.O. Box 8, 43253, Pacet-Cianjur, West Java, Indonesia aut NATIONALLICENCE 700 1- Teixeira da Silva Jaime Faculty of Agriculture and Graduate School, Kagawa University, Miki-cho, 761-0795, Kagawa, Japan aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 513-529 0167-6903 65:3<513 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer