caa a22 4500 445841745 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10725-011-9612-z doi (NATIONALLICENCE)springer-10.1007/s10725-011-9612-z Changes in cytokinin levels and metabolism in tobacco ( Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans -zeatin and dihydrozeatin [Elektronische Daten] [Marek Klemš, Zdeňka Slámová, Václav Motyka, Jiří Malbeck, Alena Trávníčková, Ivana Macháčková, Josef Holík, Stanislav Procházka] The uptake and metabolism of trans-zeatin and/or dihydrozeatin, in correlation with cytokinin oxidase/dehydrogenase (CKX) and β-glucosidase activity, were studied in leaf segments derived from wild-type (WT) and transgenic (T) tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) during in vitro induction of shoot organogenesis. T explants harbored the maize gene Zm-p60.1β-glucosidase. Higher levels of shoot regeneration were observed on T explants in the early stages of cultivation. In WT explants, the content of cytokinin (CK)-O- and N-glucosides increased. In T explants, a higher content of Z-9-riboside and Z-9-riboside-5′-monophosphate and higher CKX activity during the early stage of cultures were found. A positive correlation was obtained for bioactive CK content and the organogenic response in T explants. Our results indicate a connection between the organogenic capacity of tobacco explants, metabolism of endogenous CKs and uptake of exogenous CKs from the cultivation medium. Springer Science+Business Media B.V., 2011 β-glucosidase nationallicence Cytokinin oxidase/dehydrogenase nationallicence Dihydrozeatin nationallicence Organogenesis in vitro nationallicence trans -zeatin nationallicence BA : N 6-benzyladenine nationallicence CK : Cytokinin nationallicence CKX : Cytokinin oxidase/dehydrogenase nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence DHZ : Dihydrozeatin nationallicence DHZR : Dihydrozeatin-9-riboside nationallicence DHZRMP : Dihydrozeatin-9-riboside-5′-monophosphate nationallicence DHZOG : Dihydrozeatin-O-glucoside nationallicence DHZROG : Dihydrozeatin-9-riboside-O-glucoside nationallicence DHZ7G : Dihydrozeatin-7-glucoside nationallicence DHZ9G : Dihydrozeatin-9-glucoside nationallicence GRZ+ : Induction medium containing trans-zeatin nationallicence GRDHZ+ : Induction medium containing dihydrozeatin nationallicence GR− : Growth regulator-free medium nationallicence GRZ+/GR− : Subcultivation from zeatin induction to growth regulator-free medium nationallicence GRDHZ+/GR− : Subcultivation from dihydrozeatin induction to growth regulator-free medium nationallicence IBA : Indole-3-butyric acid nationallicence iP : N 6-(Δ2-isopentenyl)adenine nationallicence ipt : Gene coding isopentenyltransferase nationallicence iP7G : N 6-(Δ2-isopentenyl)adenine-7-glucoside nationallicence T : Transgenic tobacco nationallicence WT : Wild-type tobacco nationallicence Z : trans-zeatin nationallicence ZR : trans-zeatin-9-riboside nationallicence ZRMP : trans-zeatin-9-riboside-5′-monophosphate nationallicence ZOG : trans-zeatin-O-glucoside nationallicence Z7G : trans-zeatin-7-glucoside (abbreviations for cytokinins according to Kamínek et al. 2000) nationallicence Klemš Marek Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut Slámová Zdeňka Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut Motyka Václav Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut Malbeck Jiří Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut Trávníčková Alena Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut Macháčková Ivana Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut Holík Josef Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Procházka Stanislav Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 427-437 0167-6903 65:3<427 2011 65 10725 https://doi.org/10.1007/s10725-011-9612-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9612-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klemš Marek Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Slámová Zdeňka Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Motyka Václav Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Malbeck Jiří Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Trávníčková Alena Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Macháčková Ivana Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02, Lysolaje, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Holík Josef Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Procházka Stanislav Department of Plant Biology, Mendel University, Zemědělská 1, 613 00, Brno, Czech Republic aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 427-437 0167-6903 65:3<427 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer