caa a22 4500 445841761 CHVBK 20180317145346.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10725-011-9613-y doi (NATIONALLICENCE)springer-10.1007/s10725-011-9613-y Down-regulation of Rubisco activity under combined increases of CO2 and temperature minimized by changes in Rubisco kcat in wheat [Elektronische Daten] [Pilar Pérez, Aitor Alonso, Gloria Zita, Rosa Morcuende, Rafael Martínez-Carrasco] Increases in growth temperature have been observed to affect photosynthesis differently under long-term exposure to ambient- and twice ambient-air CO2 concentrations. This study investigates the causes of this interaction in wheat (Triticum aestivum L.) grown in the field over two consecutive years under temperature gradient chambers in ambient (370μmolmol−1) or elevated (700μmolmol−1) atmospheric CO2 concentrations and at ambient or ambient +4°C temperatures, with either a low or a high nitrogen supply. The photosynthesis-internal CO2 response curves and the activity, activation state, kcat and amount of Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) were measured, as well as the soluble protein concentration in flag leaves at ear emergence and 8-15days after anthesis. A high nitrogen supply increased Vcmax, the Rubisco amount and activity and soluble protein contents, but did not significantly change the Rubisco kcat. Both elevated CO2 and above ambient temperatures had negative effects on Vcmax and Rubisco activity, but at elevated CO2, an increase in temperature did not decrease Vcmax or Rubisco activity in relation to ambient temperature. The amounts of Rubisco and soluble protein decreased with elevated CO2 and temperature. The negative impact of elevated CO2 on Rubisco properties was somewhat counteracted at elevated temperatures by an increase in kcat. This effect can diminish the detrimental effects on photosynthesis of combined increases of CO2 and temperature. Springer Science+Business Media B.V., 2011 Triticum aestivum nationallicence Climate change nationallicence Elevated CO2 nationallicence Elevated temperature nationallicence Photosynthetic acclimation nationallicence Rubisco nationallicence Rubisco kcat nationallicence Jmax : Maximum rate of photosynthetic electron transport nationallicence RuBP : Ribulose-1, 5-bisphosphate nationallicence Vcmax : Maximum Rubisco-limited rate of carboxylation nationallicence Pérez Pilar Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut Alonso Aitor Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut Zita Gloria Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut Morcuende Rosa Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut Martínez-Carrasco Rafael Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 439-447 0167-6903 65:3<439 2011 65 10725 https://doi.org/10.1007/s10725-011-9613-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9613-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pérez Pilar Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut NATIONALLICENCE 700 1- Alonso Aitor Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut NATIONALLICENCE 700 1- Zita Gloria Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut NATIONALLICENCE 700 1- Morcuende Rosa Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut NATIONALLICENCE 700 1- Martínez-Carrasco Rafael Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Apartado 257, 37071, Salamanca, Spain aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/3(2011-12-01), 439-447 0167-6903 65:3<439 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer