caa a22 4500 445841818 CHVBK 20180317145347.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10725-011-9601-2 doi (NATIONALLICENCE)springer-10.1007/s10725-011-9601-2 Allelopathy of red pine: isolation and identification of an allelopathic substance in red pine needles [Elektronische Daten] [Hisashi Kato-Noguchi, Yoshiko Fushimi, Yukitoshi Tanaka, Toshiaki Teruya, Kiyotake Suenaga] Since red pine (Pinus densiflora Sieb. et Zucc.) often forms sparse forest floors where herbaceous plants do not grow well, allelopathy of red pine was investigated. A growth inhibitory substance was isolated from an aqueous methanol extract of red pine needles and determined by spectral data as abscisic acid-β-d-glucopyranosyl ester (ABA-GE). This substance inhibited root and shoot growth of cress and E. crus-galli seedlings at concentrations greater than 0.1μM. The concentrations required for 50% growth inhibition on roots and shoots of cress were 0.23 and 0.61μM, respectively, and those of E. crus-galli were 1.1 and 2.8μM, respectively. The activity of ABA-β-d-glucosidase, which liberates free ABA from ABA-GE, in cress and E. crus-galli seedlings was 13-29nmol mg−1 protein min−1. Endogenous concentration of ABA-GE in the pine needles was 4.1-21.5μmol kg−1 and the concentration in soil water of the pine forest was 2.5μM. The effectiveness of ABA-GE on growth inhibition and the occurrence of ABA-GE in pine needles and soil water suggest ABA-GE may play an important role in the allelopathy of red pine resulting in the formation of sparse forest floors. Springer Science+Business Media B.V., 2011 Allelopathy nationallicence Phytoalexin nationallicence Pinaceae nationallicence Pine forest nationallicence Growth inhibitor nationallicence ABA-GE : Abscisic acid-β-d-glucopyranosyl ester nationallicence Kato-Noguchi Hisashi Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Fushimi Yoshiko Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Tanaka Yukitoshi Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut Teruya Toshiaki Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, 223-8522, Kohoku, Yokohama, Japan aut Suenaga Kiyotake Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, 223-8522, Kohoku, Yokohama, Japan aut Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 299-304 0167-6903 65:2<299 2011 65 10725 https://doi.org/10.1007/s10725-011-9601-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-011-9601-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato-Noguchi Hisashi Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Fushimi Yoshiko Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Tanaka Yukitoshi Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 761-0795, Miki, Kagawa, Japan aut NATIONALLICENCE 700 1- Teruya Toshiaki Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, 223-8522, Kohoku, Yokohama, Japan aut NATIONALLICENCE 700 1- Suenaga Kiyotake Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, 223-8522, Kohoku, Yokohama, Japan aut NATIONALLICENCE 773 0- Plant Growth Regulation Springer Netherlands 65/2(2011-11-01), 299-304 0167-6903 65:2<299 2011 65 10725 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer